Optical configuration of the Influx
| Influx optics | |||
|---|---|---|---|
| Laser | Band pass filter | Detector name | Fluorophores used in this study |
| 405 nm | 460/50 | BV421 | Hoechst 33342, Violet amine-reactive dye |
| 488 nm | 488/10 | SSC | |
| 488 nm | 530/40 | FITC | Alexa Fluor 488, Calcein AM |
| 488 nm | 692/40 | PerCP-Cy5.5 | FM4-64 |
| 561 nm | 589/29 | PE | Alexa Fluor 555, Calcein red |
| 638 nm | 670/30 | APC | Alexa Fluor 647, MitoTracker Deep Red FM |