Optical configuration of the LSRFortessa
| LSRFortessa optics | ||||
|---|---|---|---|---|
| Laser | Band pass filter | Dichroic filter | Detector name | Fluorophores used in this study |
| 405 nm | 450/50 | Blank | Pacific Blue | Violet amine-reactive dye |
| 488 nm | 488/10 | Blank | SSC | |
| 488 nm | 530/30 | 505LP | FITC | Alexa Fluor 488, Calcein AM |
| 488 nm | 710/50 | 685LP | PerCP-Cy5.5 | FM4-64 |
| 561 nm | 582/12 | Blank | PE | Alexa Fluor 555, Calcein red |
| 633 nm | 670/30 | Blank | APC | Alexa Fluor 647, MitoTracker Deep Red FM |