Expression of c-Fos measured using RT-qPCR and IHC
RT-qPCR for 5-HT activation experiment (Fig. 4B) | Behavioral test for control, unpaired trained and paired trained snails using CS or DS (approach time latency in seconds; Fig. 4D) | ||||||
---|---|---|---|---|---|---|---|
Control | Stimulated | Control CS | Control DS | Unpaired CS | Unpaired DS | Trained CS | Trained DS |
5.53 | 2.99 | 23.3 | 60.7 | 36.7 | 42 | 112.7 | 45.3 |
2.73 | 6.5 | 58.7 | 57.7 | 57.7 | 33.7 | 112.7 | 54 |
1.11 | NA | 45.3 | 41.3 | 41.3 | 28.7 | 83 | 31.3 |
0.24 | 3.21 | 26 | 64.3 | 49.3 | 51.7 | 109 | 38.7 |
0.25 | 6.03 | 36.7 | 34 | 16.7 | 39 | 90.7 | 60.7 |
37.3 | 40.3 | 44.7 | 25.3 | 120 | 56.3 | ||
23.3 | 28.7 | 54 | 51 | 102.7 | 66.7 | ||
Fluorescence intensity of c-Fos in Pa2 2 h after training (Fig. 4F) | Fluorescence intensity of c-Fos in Pa3 after unilateral stimulation (Fig. 4F) | Fluorescence intensity of c-Fos in Pa2 2 h after training (Fig. 4H) | Fluorescence intensity of c-Fos in serotoninergic neurons 2 h after training (Fig. 4H) | ||||
Control | Trained | Stimulated | Control | Unpaired | Paired | Unpaired | Paired |
283.3 | 819.7 | 735.7 | 284.7 | 603.3 | 1274.7 | 355.7 | 1789 |
332.7 | 1223 | 976 | 38.7 | 1449.3 | 536.3 | 766.3 | 1344 |
71.3 | 470.3 | 637 | 310 | 1181.7 | 736 | 459 | 1511 |
131.7 | 698.3 | 498.3 | 214.3 | 347.7 | 582.7 | 788.3 | 1329.7 |
328.7 | 1100.3 | 844.7 | 58.7 | 352.7 | 339.3 | 436.3 | 1082 |