Key resources
| Reagent or resource | Source | Identifier |
|---|---|---|
| Antibodies | ||
| Anti-GFP (green fluorescent protein; chicken antibodies, IgY fraction) | Aves | GFP-1020 |
| Rabbit mCherry | Invitrogen | PA5-34974 |
| Streptavidin, Alexa Fluor 647 conjugate | Invitrogen | S32357 |
| Alexa Fluor 488 goat anti-chicken (H + L) | Life Technologies | A11039 |
| Alexa Fluor 568 goat anti-rabbit IgG (H + L) | Life Technologies | P36971 |
| Plasmids | ||
| pRubi | Luikart Lab (Dartmouth) | |
| redRubi | Luikart Lab (Dartmouth) | |
| Chemicals, media | ||
| ProLong diamond antifade mountant with DAPI | Invitrogen | P36971 |
| Iscove's modification of DMEM | Corning | 10-016-CV |
| L-glutamine, 100×, liquid | Corning | 25-005-CI |
| MEM nonessential amino acids | Corning | 25-025-CI |
| Penicillin-streptomycin solution, 100× | Corning | 30-002-CI |
| PEG 6000, molecular biology grade | Millipore Sigma | 528877 |
| L-15 medium (Leibovitz) with L-glutamine | Sigma-Aldrich | SLBR4210V |
| Defined trypsin inhibitor (1×) | Gibco | R-007-100 |
| 2.5% trypsin (10×) | Gibco | 15090-046 |
| Mouse EGF | Cell Signaling | 5331SF |
| Fibroblast growth factor-basic human | Cell Signaling | F0291 |
| B-27 supplement | Gibco | 17504-044 |
| Caspase inhibitor Z-VAD-FMK 20 mM | Promega | G7231 |
| Commercial kits | ||
| NucleoBond Xtra Maxi DNA, RNA and protein purification kit | Macherey-Nagel | 740414.10 |
| Experimental models: cell lines | ||
| 293 GP | Luikart Lab (Dartmouth) | |
| 293 R | Luikart Lab (Dartmouth) | |
| Experimental models: mice | ||
| C57BL/NHsd | Envigo | |
| B6.Cg-Tg(Slc32a1-COP4*H134R/EYFP)8Gfng/J (VGAT-ChR2-EYFP line 8) | The Jackson Laboratory | 014548 |
| Software IMARIS | Bitplane | |