Gene | Primers | Amplicon | Efficiency | Amplicon Tmelt (°C) |
---|---|---|---|---|
actb* | Forward, GTTGATAATGGATCTGGTATGTGC | Nucleotides 89–201, GenBank accession number NM_001088953.1 | 0.9401 | 83.0 |
Reverse, ATTCCAACCATGACACCCTGA | ||||
dynll1◊ | Forward, TGCTACTCAGGCACTGGAGA | Nucleotides 127–239, GenBank accession number NM_001171695.1 | 0.9576 | 77.5 |
Reverse, AATTCCTTCCCACAATGCAA | ||||
sema3a | Forward, TGAAGAACGGGGAAGACTTTATG | Nucleotides 189–288, GenBank accession number NM_001085855.1 | 0.9738 | 78.5 |
Reverse, TGTTACAGGCCACAATATCTTTTG | ||||
slit1* | Forward, TGCTGAGCGTAAACTTTGTGG | Nucleotides 3756–3855, GenBank accession number NM_001087109.1 | 0.9931 | 81.0 |
Reverse, TCCGCTGTTGACACCTGAAG | ||||
slit2 | Forward, CCAGGAGCATTCTCACCATACA | Nucleotides 1510–1628, GenBank accession number NM_001087668.1 | 0.9220 | 80.0 |
Reverse, TAGAGAACAAGGGAGTTGAGTGA | ||||
spry1 a/b* | Forward, CAACATGGCATTGGTGGTTCAT | Nucleotides 16–110, GenBank accession number NM_001137585.1; nucleotides 72–166, GenBank accession number NM_001159681.1 | 0.9490 | 82.0 |
Reverse, TTTGATCTGATCCAAGGACAAGATAG | ||||
tubb* | Forward, GACCCTTTGGACAGATTTTCAGG | Nucleotides 300–405, GenBank accession number NM_001087257.1 | 0.9635 | 81.5 |
*Yang et al. (2018).
◊Brunsdon (2015).