Figure/table | Data structure | Type of test | Sample size | Statistical data |
---|---|---|---|---|

Fig. 1A | ||||

Ethanol consumption | ||||

WT and SA/SA mice (5%) | Normal distribution | Welch t test unpaired | WT: n = 6, SA/SA: n = 7 | CI(d): –2.681 and –0.273, t = –2.867, p = 0.0226 |

WT and SA/SA mice (10%) | Normal distribution | Welch t test unpaired | WT: n = 5, SA/SA: n = 5 | CI(d): –1.730 and –0.258, t = –3.562, p = 0.0184 |

WT and SD/SD mice (5%) | Normal distribution | Welch t test unpaired | WT: n = 7, SD/SD: n = 6 | CI(d): –1.705 and 0.765, t = –0.906, p = 0.3960 |

WT and SD/SD mice (10%) | Non-normal distribution | Mann–Whitney U test | WT: n = 8, SD/SD: n = 8 | U = 26, p = 0.5286 |

Fig. 1B | ||||

Ethanol preference ratio | ||||

WT and SA/SA mice (5%) | Normal distribution | Welch t test unpaired | WT: n = 6, SA/SA: n = 7 | CI(d): –0.444 and –0.056, t = –2.987, p = 0.0179 |

WT and SA/SA mice (10%) | Normal distribution | Welch t test unpaired | WT: n = 5, SA/SA: n = 5 | CI(d): –0.177 and –0.039, t = –3.724, p = 0.0080 |

WT and SD/SD mice (5%) | Normal distribution | Welch t test unpaired | WT: n = 7, SD/SD: n = 6 | CI(d): –0.311 and 0.187, t = –0.588, p = 0.5747 |

WT and SD/SD mice (10%) | Normal distribution | Welch t test unpaired | WT: n = 8, SD/SD: n = 8 | CI(d): –0.109 and 0.094, t = –0.155, p = 0.8791 |

Fig. 1C | ||||

Total liquid intake | ||||

WT and SA/SA mice (5%) | Normal distribution | Welch t test unpaired | WT: n = 6, SA/SA: n = 7 | CI(d): –0.130 and 0.296, t = 0.916, p = 0.3900 |

WT and SA/SA mice (10%) | Normal distribution | Welch t test unpaired | WT: n = 5, SA/SA: n = 5 | CI(d): –0.156 and 0.133, t = –0.190, p = 0.8546 |

WT and SD/SD mice (5%) | Normal distribution | Welch t test unpaired | WT: n = 7, SD/SD: n = 6 | CI(d): –0.272 and 0.117, t = –0.877, p = 0.3999 |

WT and SD/SD mice (10%) | Normal distribution | Welch t test unpaired | WT: n = 8, SD/SD: n = 8 | CI(d): –0.223 and 0.191, t = –0.166, p = 0.8712 |

Fig. 2A | ||||

Taste preference ratio (WT and SA/SA) | ||||

Sucrose | Normal distribution | Paired factorial ANOVA | WT: n = 5, SA/SA: n = 5 | Genotype: F = 0.5107, p = 0.4951 |

0.033% (w/v) | Bonferroni’s post hoc | CI(m): WT, 0.477 and 0.569; SA/SA, 0.489 and 0.580, p = 0.5901 | ||

0.1% (w/v) | Bonferroni’s post hoc | CI(m): WT, 0.430 and 0.521; SA/SA, 0.447 and 0.538, p = 0.4224 | ||

Quinine | Normal distribution | Paired factorial ANOVA | WT: n = 5, SA/SA: n = 5 | Genotype: F = 0.0331, p = 0.8602 |

0.02 mM | Bonferroni’s post hoc | CI(m): WT, 0.134 and 0.522; SA/SA, 0.139 and 0.527, p = 0.9484 | ||

0.04 mM | Bonferroni’s post hoc | CI(m): WT, 0.033 and 0.421; SA/SA, 0.060 and 0.448, p = 0.6783 | ||

Fig. 2B | ||||

Taste preference ratio (WT and SD/SD) | ||||

Sucrose | Normal distribution | Paired factorial ANOVA | WT: n = 5, SD/SD: n = 5 | Genotype: F = 0.8475, p = 0.3842 |

0.033% (w/v) | Bonferroni’s post hoc | CI(m): WT, 0.495 and 0.604; SD/SD, 0.447 and 0.557, p = 0.0533 | ||

0.1% (w/v) | Bonferroni’s post hoc | CI(m): WT, 0.518 and 0.627; SD/SD, 0.522 and 0.631, p = 0.8722 | ||

Quinine | Normal distribution | Paired factorial ANOVA | WT: n = 5, SD/SD: n = 5 | Genotype: F = 0.4920, p = 0.5029 |

0.02 mM | Bonferroni’s post hoc | CI(m): WT, 0.231 and 0.540; SD/SD, 0.190 and 0.499, p = 0.4454 | ||

0.04 mM | Bonferroni’s post hoc | CI(m): WT, 0.056 and 0.365; SD/SD, 0.003 and 0.312, p = 0.3294 | ||

Fig. 2C | ||||

Total sucrose intake | ||||

WT and SA/SA mice | Normal distribution | Paired factorial ANOVA | WT: n = 5, SA/SA: n = 5 | Genotype: F = 0.3578, p = 0.5663 |

0.033% (w/v) | Bonferroni’s post hoc | CI(m): WT, 0.442 and 1.558; SA/SA, 0.578 and 1.694, p = 0.4868 | ||

0.1% (w/v) | Bonferroni’s post hoc | CI(m): WT, 2.105 and 3.221; SA/SA, 2.258 and 3.375, p = 0.4345 | ||

WT and SD/SD mice | Normal distribution | Paired factorial ANOVA | WT: n = 5, SD/SD: n = 5 | Genotype: F = 0.2553, p = 0.6270 |

0.033% (w/v) | Bonferroni’s post hoc | CI(m): WT, 0.442 and 1.558; SD/SD, 0.214 and 1.329, p = 0.2855 | ||

0.1% (w/v) | Bonferroni’s post hoc | CI(m): WT, 2.359 and 3.475; SD/SD, 2.343 and 3.458, p = 0.9385 | ||

Fig. 3 | ||||

Blood ethanol concentration | ||||

WT and SA/SA mice | Normal distribution | Paired factorial ANOVA | WT: n = 6, SA/SA: n = 5 | Genotype: F = 0.7817, p = 0.3996 |

1 h | Tukey–Kramer post hoc | CI(m): WT, 2085.307 and 2908.035; SA/SA, 2108.846 and 3010.099, p = 0.6879 | ||

3 h | Tukey–Kramer post hoc | CI(m): WT, 1063.610 and 1886.337; SA/SA, 1298.783 and 2200.036, p = 0.0947 | ||

WT and SD/SD mice | Normal distribution | Paired factorial ANOVA | WT: n = 5, SD/SD: n = 4 | Genotype: F = 0.1381, p = 0.7212 |

1 h | Tukey–Kramer post hoc | CI(m): WT, 2115.538 and 3687.719; SD/SD, 2109.947 and 3867.699, p = 0.7418 | ||

3 h | Tukey–Kramer post hoc | CI(m): WT, 1211.065 and 2783.246; SD/SD, 769.016 and 2526.767, p = 0.2089 | ||

Fig. 4 | ||||

LORR | ||||

WT and SA/SA mice | Normal distribution | Welch t test unpaired | WT: n = 13, SA/SA: n = 13 | CI(d): –12.835 and 11.450, t = –0.118, p = 0.9072 |

WT and SD/SD mice | Normal distribution | Welch t test unpaired | WT: n = 16, SD/SD: n = 13 | CI(d): –6.790 and 28.367, t = 1.261, p = 0.2184 |

Fig. 5A | ||||

ROCK activity | ||||

WT and SA/SA mice | Assuming normality | Welch t test unpaired | WT: n = 4, SA/SA: n = 4 | CI(d): 18.691 and 158.488, t = 3.305, p = 0.0228 |

WT and SD/SD mice | Assuming normality | Welch t test unpaired | WT: n = 4, SD/SD: n = 4 | CI(d): –157.109 and 115.935, t = –0.421, p = 0.6956 |

Fig. 5B | ||||

ROCK protein level | ||||

WT and SA/SA mice | Assuming normality | Welch t test unpaired | WT: n = 4, SA/SA: n = 4 | CI(d): –0.015 and 0.014, t = –0.141, p = 0.8929 |

WT and SD/SD mice | Assuming normality | Welch t test unpaired | WT: n = 4, SD/SD: n = 4 | CI(d): –0.032 and 0.085, t = 1.248, p = 0.2782 |

Fig. 5C | ||||

ROCK activity/protein. | ||||

WT and SA/SA mice | Assuming normality | Welch t test unpaired | WT: n = 4, SA/SA: n = 4 | CI(d): 194.255 and 925.975, t = 3.747, p = 0.0096 |

WT and SD/SD mice | Assuming normality | Welch t test unpaired | WT: n = 4, SD/SD: n = 4 | CI(d): –1286.927 and 604.178, t = –0.937, p = 0.3929 |

Fig. 5D | ||||

Phosphorylated Akt | ||||

WT and SA/SA mice | Normal distribution | Welch t test unpaired | WT: n = 5, SA/SA: n = 5 | CI(d): –0.077 and –0.007, t = –2.751, p = 0.0256 |

Table 2 | ||||

Body weight | ||||

Fig. 1 (ethanol two-bottle choice test) | ||||

WT and SA/SA mice (5%) | Normal distribution | Welch t test unpaired | WT: n = 6, SA/SA: n = 7 | CI(d): –0.003 and 0.002, t = –0.512, p = 0.6229 |

WT and SA/SA mice (10%) | Normal distribution | Welch t test unpaired | WT: n = 5, SA/SA: n = 5 | CI(d): –0.002 and 0.009, t = 1.561, p = 0.1651 |

WT and SD/SD mice (5%) | Normal distribution | Welch t test unpaired | WT: n = 7, SD/SD: n = 6 | CI(d): –0.001 and 0.007, t = 1.907, p = 0.0874 |

WT and SD/SD mice (10%) | Normal distribution | Welch t test unpaired | WT: n = 8, SD/SD: n = 8 | CI(d): –0.003 and 0.003, t = 0.029, p = 0.9770 |

Fig. 2 (taste preference test) | ||||

WT/WT and SA/SA mice | Normal distribution | Welch t test unpaired | WT: n = 5, SA/SA: n = 5 | CI(d): –0.005 and 0.007, t = 0.435, p = 0.6769 |

WT/WT and SD/SD mice | Normal distribution | Welch t test unpaired | WT: n = 5, SA/SA: n = 5 | CI(d): –0.004 and 0.009, t = 0.847, p = 0.4226 |

Table 3 | ||||

Putative average ethanol consumption | ||||

WT and SA/SA mice | ||||

5% ethanol | Normal distribution | Welch t test unpaired | WT: n = 6, SA/SA: n = 7 | CI(d): –2.223 and –0.209, t = –2.809, p = 0.0240 |

10% ethanol | Normal distribution | Welch t test unpaired | WT: n = 5, SA/SA: n = 5 | CI(d): –1.707 and –0.305, t = –3.309, p = 0.0107 |

WT and SD/SD mice | ||||

5% ethanol | Normal distribution | Welch t test unpaired | WT: n = 7, SD/SD: n = 6 | CI(d): –1.692 and 0.833, t = –0.820, p = 0.4414 |

10% ethanol | Non-normal distribution | Mann–Whitney U test | WT: n = 8, SD/SD: n = 8 | U = 26, p = 0.5286 |

Table 4 | ||||

Putative average total liquid intake | ||||

WT and SA/SA mice | ||||

5% ethanol | Normal distribution | Welch t test unpaired | WT: n = 6, SA/SA: n = 7 | CI(d): –19.2958 and 42.1470, t = 0.8822, p = 0.4074 |

10% ethanol | Normal distribution | Welch t test unpaired | WT: n = 5, SA/SA: n = 5 | CI(d): –12.7726 and 19.1717, t = 0.4800, p = 0.6468 |

WT and SD/SD mice | ||||

5% ethanol | Normal distribution | Welch t test unpaired | WT: n = 7, SD/SD: n = 6 | CI(d): –29.0687 and 14.5159, t = –0.7377, p = 0.4766 |

10% ethanol | Normal distribution | Welch t test unpaired | WT: n = 8, SD/SD: n = 8 | CI(d): –25.3723 and 22.8610, t = –0.1142, p = 0.9111 |

CI(d), 95% confidence interval for the difference in population means, lower, and upper limits; CI(m), 95% confidence interval for the population means, lower, and upper limits.