Table 1.

Statistical table

Figure/table	Data structure	Type of test	Sample size	Statistical data
Fig. 1A
Ethanol consumption
WT and SA/SA mice (5%)	Normal distribution	Welch t test unpaired	WT: n = 6, SA/SA: n = 7	CI(d): –2.681 and –0.273, t = –2.867, p = 0.0226
WT and SA/SA mice (10%)	Normal distribution	Welch t test unpaired	WT: n = 5, SA/SA: n = 5	CI(d): –1.730 and –0.258, t = –3.562, p = 0.0184
WT and SD/SD mice (5%)	Normal distribution	Welch t test unpaired	WT: n = 7, SD/SD: n = 6	CI(d): –1.705 and 0.765, t = –0.906, p = 0.3960
WT and SD/SD mice (10%)	Non-normal distribution	Mann–Whitney U test	WT: n = 8, SD/SD: n = 8	U = 26, p = 0.5286
Fig. 1B
Ethanol preference ratio
WT and SA/SA mice (5%)	Normal distribution	Welch t test unpaired	WT: n = 6, SA/SA: n = 7	CI(d): –0.444 and –0.056, t = –2.987, p = 0.0179
WT and SA/SA mice (10%)	Normal distribution	Welch t test unpaired	WT: n = 5, SA/SA: n = 5	CI(d): –0.177 and –0.039, t = –3.724, p = 0.0080
WT and SD/SD mice (5%)	Normal distribution	Welch t test unpaired	WT: n = 7, SD/SD: n = 6	CI(d): –0.311 and 0.187, t = –0.588, p = 0.5747
WT and SD/SD mice (10%)	Normal distribution	Welch t test unpaired	WT: n = 8, SD/SD: n = 8	CI(d): –0.109 and 0.094, t = –0.155, p = 0.8791
Fig. 1C
Total liquid intake
WT and SA/SA mice (5%)	Normal distribution	Welch t test unpaired	WT: n = 6, SA/SA: n = 7	CI(d): –0.130 and 0.296, t = 0.916, p = 0.3900
WT and SA/SA mice (10%)	Normal distribution	Welch t test unpaired	WT: n = 5, SA/SA: n = 5	CI(d): –0.156 and 0.133, t = –0.190, p = 0.8546
WT and SD/SD mice (5%)	Normal distribution	Welch t test unpaired	WT: n = 7, SD/SD: n = 6	CI(d): –0.272 and 0.117, t = –0.877, p = 0.3999
WT and SD/SD mice (10%)	Normal distribution	Welch t test unpaired	WT: n = 8, SD/SD: n = 8	CI(d): –0.223 and 0.191, t = –0.166, p = 0.8712
Fig. 2A
Taste preference ratio (WT and SA/SA)
Sucrose	Normal distribution	Paired factorial ANOVA	WT: n = 5, SA/SA: n = 5	Genotype: F = 0.5107, p = 0.4951
0.033% (w/v)		Bonferroni’s post hoc		CI(m): WT, 0.477 and 0.569; SA/SA, 0.489 and 0.580, p = 0.5901
0.1% (w/v)		Bonferroni’s post hoc		CI(m): WT, 0.430 and 0.521; SA/SA, 0.447 and 0.538, p = 0.4224
Quinine	Normal distribution	Paired factorial ANOVA	WT: n = 5, SA/SA: n = 5	Genotype: F = 0.0331, p = 0.8602
0.02 mM		Bonferroni’s post hoc		CI(m): WT, 0.134 and 0.522; SA/SA, 0.139 and 0.527, p = 0.9484
0.04 mM		Bonferroni’s post hoc		CI(m): WT, 0.033 and 0.421; SA/SA, 0.060 and 0.448, p = 0.6783
Fig. 2B
Taste preference ratio (WT and SD/SD)
Sucrose	Normal distribution	Paired factorial ANOVA	WT: n = 5, SD/SD: n = 5	Genotype: F = 0.8475, p = 0.3842
0.033% (w/v)		Bonferroni’s post hoc		CI(m): WT, 0.495 and 0.604; SD/SD, 0.447 and 0.557, p = 0.0533
0.1% (w/v)		Bonferroni’s post hoc		CI(m): WT, 0.518 and 0.627; SD/SD, 0.522 and 0.631, p = 0.8722
Quinine	Normal distribution	Paired factorial ANOVA	WT: n = 5, SD/SD: n = 5	Genotype: F = 0.4920, p = 0.5029
0.02 mM		Bonferroni’s post hoc		CI(m): WT, 0.231 and 0.540; SD/SD, 0.190 and 0.499, p = 0.4454
0.04 mM		Bonferroni’s post hoc		CI(m): WT, 0.056 and 0.365; SD/SD, 0.003 and 0.312, p = 0.3294
Fig. 2C
Total sucrose intake
WT and SA/SA mice	Normal distribution	Paired factorial ANOVA	WT: n = 5, SA/SA: n = 5	Genotype: F = 0.3578, p = 0.5663
0.033% (w/v)		Bonferroni’s post hoc		CI(m): WT, 0.442 and 1.558; SA/SA, 0.578 and 1.694, p = 0.4868
0.1% (w/v)		Bonferroni’s post hoc		CI(m): WT, 2.105 and 3.221; SA/SA, 2.258 and 3.375, p = 0.4345
WT and SD/SD mice	Normal distribution	Paired factorial ANOVA	WT: n = 5, SD/SD: n = 5	Genotype: F = 0.2553, p = 0.6270
0.033% (w/v)		Bonferroni’s post hoc		CI(m): WT, 0.442 and 1.558; SD/SD, 0.214 and 1.329, p = 0.2855
0.1% (w/v)		Bonferroni’s post hoc		CI(m): WT, 2.359 and 3.475; SD/SD, 2.343 and 3.458, p = 0.9385
Fig. 3
Blood ethanol concentration
WT and SA/SA mice	Normal distribution	Paired factorial ANOVA	WT: n = 6, SA/SA: n = 5	Genotype: F = 0.7817, p = 0.3996
1 h		Tukey–Kramer post hoc		CI(m): WT, 2085.307 and 2908.035; SA/SA, 2108.846 and 3010.099, p = 0.6879
3 h		Tukey–Kramer post hoc		CI(m): WT, 1063.610 and 1886.337; SA/SA, 1298.783 and 2200.036, p = 0.0947
WT and SD/SD mice	Normal distribution	Paired factorial ANOVA	WT: n = 5, SD/SD: n = 4	Genotype: F = 0.1381, p = 0.7212
1 h		Tukey–Kramer post hoc		CI(m): WT, 2115.538 and 3687.719; SD/SD, 2109.947 and 3867.699, p = 0.7418
3 h		Tukey–Kramer post hoc		CI(m): WT, 1211.065 and 2783.246; SD/SD, 769.016 and 2526.767, p = 0.2089
Fig. 4
LORR
WT and SA/SA mice	Normal distribution	Welch t test unpaired	WT: n = 13, SA/SA: n = 13	CI(d): –12.835 and 11.450, t = –0.118, p = 0.9072
WT and SD/SD mice	Normal distribution	Welch t test unpaired	WT: n = 16, SD/SD: n = 13	CI(d): –6.790 and 28.367, t = 1.261, p = 0.2184
Fig. 5A
ROCK activity
WT and SA/SA mice	Assuming normality	Welch t test unpaired	WT: n = 4, SA/SA: n = 4	CI(d): 18.691 and 158.488, t = 3.305, p = 0.0228
WT and SD/SD mice	Assuming normality	Welch t test unpaired	WT: n = 4, SD/SD: n = 4	CI(d): –157.109 and 115.935, t = –0.421, p = 0.6956
Fig. 5B
ROCK protein level
WT and SA/SA mice	Assuming normality	Welch t test unpaired	WT: n = 4, SA/SA: n = 4	CI(d): –0.015 and 0.014, t = –0.141, p = 0.8929
WT and SD/SD mice	Assuming normality	Welch t test unpaired	WT: n = 4, SD/SD: n = 4	CI(d): –0.032 and 0.085, t = 1.248, p = 0.2782
Fig. 5C
ROCK activity/protein.
WT and SA/SA mice	Assuming normality	Welch t test unpaired	WT: n = 4, SA/SA: n = 4	CI(d): 194.255 and 925.975, t = 3.747, p = 0.0096
WT and SD/SD mice	Assuming normality	Welch t test unpaired	WT: n = 4, SD/SD: n = 4	CI(d): –1286.927 and 604.178, t = –0.937, p = 0.3929

Fig. 5D
Phosphorylated Akt
WT and SA/SA mice	Normal distribution	Welch t test unpaired	WT: n = 5, SA/SA: n = 5	CI(d): –0.077 and –0.007, t = –2.751, p = 0.0256
Table 2
Body weight
Fig. 1 (ethanol two-bottle choice test)
WT and SA/SA mice (5%)	Normal distribution	Welch t test unpaired	WT: n = 6, SA/SA: n = 7	CI(d): –0.003 and 0.002, t = –0.512, p = 0.6229
WT and SA/SA mice (10%)	Normal distribution	Welch t test unpaired	WT: n = 5, SA/SA: n = 5	CI(d): –0.002 and 0.009, t = 1.561, p = 0.1651
WT and SD/SD mice (5%)	Normal distribution	Welch t test unpaired	WT: n = 7, SD/SD: n = 6	CI(d): –0.001 and 0.007, t = 1.907, p = 0.0874
WT and SD/SD mice (10%)	Normal distribution	Welch t test unpaired	WT: n = 8, SD/SD: n = 8	CI(d): –0.003 and 0.003, t = 0.029, p = 0.9770
Fig. 2 (taste preference test)
WT/WT and SA/SA mice	Normal distribution	Welch t test unpaired	WT: n = 5, SA/SA: n = 5	CI(d): –0.005 and 0.007, t = 0.435, p = 0.6769
WT/WT and SD/SD mice	Normal distribution	Welch t test unpaired	WT: n = 5, SA/SA: n = 5	CI(d): –0.004 and 0.009, t = 0.847, p = 0.4226
Table 3
Putative average ethanol consumption
WT and SA/SA mice
5% ethanol	Normal distribution	Welch t test unpaired	WT: n = 6, SA/SA: n = 7	CI(d): –2.223 and –0.209, t = –2.809, p = 0.0240
10% ethanol	Normal distribution	Welch t test unpaired	WT: n = 5, SA/SA: n = 5	CI(d): –1.707 and –0.305, t = –3.309, p = 0.0107
WT and SD/SD mice
5% ethanol	Normal distribution	Welch t test unpaired	WT: n = 7, SD/SD: n = 6	CI(d): –1.692 and 0.833, t = –0.820, p = 0.4414
10% ethanol	Non-normal distribution	Mann–Whitney U test	WT: n = 8, SD/SD: n = 8	U = 26, p = 0.5286
Table 4
Putative average total liquid intake
WT and SA/SA mice
5% ethanol	Normal distribution	Welch t test unpaired	WT: n = 6, SA/SA: n = 7	CI(d): –19.2958 and 42.1470, t = 0.8822, p = 0.4074
10% ethanol	Normal distribution	Welch t test unpaired	WT: n = 5, SA/SA: n = 5	CI(d): –12.7726 and 19.1717, t = 0.4800, p = 0.6468
WT and SD/SD mice
5% ethanol	Normal distribution	Welch t test unpaired	WT: n = 7, SD/SD: n = 6	CI(d): –29.0687 and 14.5159, t = –0.7377, p = 0.4766
10% ethanol	Normal distribution	Welch t test unpaired	WT: n = 8, SD/SD: n = 8	CI(d): –25.3723 and 22.8610, t = –0.1142, p = 0.9111

CI(d), 95% confidence interval for the difference in population means, lower, and upper limits; CI(m), 95% confidence interval for the population means, lower, and upper limits.