List of figures for each experiment indicating data structure, statistical tests applied, and significance levels
| Figure | Data structure | Type of test | Power | Notes |
|---|---|---|---|---|
| Fig. 1C–E | GSEA | Kolmogorov–Smirnov | FDR < 0.01 | |NES| > 2 |
| Fig. 2A,B | Differential expression analysis | DESeq2 generalized linear model (GLM) | FDR < 0.1 | FPKM > 1, normalized count fold-change > 2 |
| Fig. 2C | Gene set enrichment | Hypergeometric | FDR < 0.05 | Gene no. > 5 |
| Fig. 2D | Differential expression analysis | DESeq2 GLM | p < 0.05 | FPKM > 1, normalized counts, fold-change >4 (comparedwith astrocyte subpopulations from other regions),fold-change > 2 (compared with non-astrocyte sample). |
| Fig. 2-2 | Normal distribution | Unpaired Student’s t test | p < 0.05 | |
| Figs. 3C, 4B | Correlations | ANOVA, Wilcoxon rank sum tests | p < 0.05 (FDR < 0.25) | |
| Fig. 5 | Kaplan–Meier survival plots | Log rank test Fisher’s test | p < 0.05 | |
| Fig. 6A–C, Fig. 6-2B–D | Immunostained images | Two-tailed unpaired Student’s t test | p < 0.05, p < 0.01, p < 0.001 |