ChIP-qPCR Primers along the Fosb gene
| Location on Fosb | Forward | Reverse |
|---|---|---|
| −1250 | ATGGGACTCAGGTTGTCAGG | AGCCAGGGCTACACAGAGAA |
| −500 | GAGTTGCACCTTCTCCAACC | GGCCCAGTGTTTGTTTGGTA |
| −250 | ATGGCTAATTGCGTCACAGG | ACCTCCCAAACTCTCCCTTC |
| Exon IV | CAACCTGACGGCTTCTCTCT | CGGGTTTGTTTGTTTTGTTTG |
These qChIP primers were used to amplify three putative E2F consensus sequences in Fosb: 500 and 250 bp upstream of the TSS, and a site within Exon IV. A sequence 1250 bp upstream of the TSS was used as a negative control.