RT-qPCR Primers for Fosb transcripts and Gapdh for probing mRNA expression
| Transcript | Forward | Reverse |
|---|---|---|
| Fosb | GTGAGAGATTTGCCAGGGTC | AGAGAGAAGCCGTCAGGTTG |
| ΔFosb | AGGCAGAGCTGGAGTCGGAGAT | GCCGAGGACTTGAACTTCACTCG |
| Gapdh | AGGTCGGTGTGAACGGATTTG | TGTAGACCATGTAGTTGAGGTCA |
These qPCR primers were used to amplify and assess expression of Fosb and ΔFosb mRNA. Gapdh was used as a housekeeping gene in the ΔΔCt method.