A KS2D2S test to identify differences between frazzled LOF mutants and LOF flies driving different UAS-Frazzled rescue constructs
| KS2D2S | |||||
|---|---|---|---|---|---|
| Sample 1 (n = 35 terminals) | Sample 2 | Sample 2 Terminals | p-value (Response Latency versus GJ%) | p-value (Response Frequency versus GJ%) | |
| 1 | frazzled LOF mutant (w; fra3/fra4; R91H05::GFP/+) | frazzled heterozygous sibling (w; fra3/CyO; R91H05::GFP/+) | n = 16 | 0.0001* | 0.0001* |
| 2 | frazzled LOF mutant (w; fra3/fra4; R91H05::GFP/+) | Full-length Frazzled (w; fra3/fra4; R91H05::GFP/UAS-Frazzled) | n = 38 | 0.0373* | 0.0076* |
| 3 | frazzled LOF mutant (w; fra3/fra4; R91H05::GFP/+) | Frazzled’s intracellular domain (w; fra3/fra4; R91H05::GFP/UAS-FraICD) | n = 23 | 0.0007* | 0.0004* |
| 4 | frazzled LOF mutant (w; fra3/fra4; R91H05::GFP/+) | Point-mutated Frazzled (w; fra3/fra4; R91H05::GFP/UAS-HA-FraE1354A) | n = 13 | 0.0628 | 0.0404* |
| 5 | frazzled LOF mutant (w; fra3/fra4; R91H05::GFP/+) | Frazzled P1 domain deletion (w; fra3/fra4; R91H05::GFP/UAS-HA-FraΔP1) | n = 7 | 0.0013* | 0.0153* |
| 6 | frazzled LOF mutant (w; fra3/fra4; R91H05::GFP/+) | Frazzled P2 domain deletion (w; fra3/fra4; R91H05::GFP/UAS-HA-FraΔP2) | n = 20 | 0.0002* | 0.0054* |
| 7 | frazzled LOF mutant (w; fra3/fra4; R91H05::GFP/+) | Frazzled P3 domain deletion (w; fra3/fra4; R91H05::GFP/UAS-HA-FraΔP3) | n = 12 | 0.3085 | 0.5607 |
| 8 | frazzled LOF mutant (w; fra3/fra4; R91H05::GFP/+) | Wild-type unablated (w; + ; R91H05::GFP/10×-UAS-GFP) | n = 8 | 0.0055* | 0.0098* |
| 9 | frazzled LOF mutant (w; fra3/fra4; R91H05::GFP/+) | Wild-type ablated (w; + ; R91H05::GFP/10×-UAS-GFP) | n = 22 | 0.0282* | 0.0009* |
| 10 | frazzled LOF mutant (w; fra3/fra4; R91H05::GFP/+) | shak-B2 heterozygous sibling ablation (shak-B2/Fm6; + ; R91H05::GFP/R91H05::GFP) | n = 14 | 0.3688* | 0.0076* |
| 11 | frazzled LOF mutant (w; fra3/fra4; R91H05::GFP/+) | shak-B2 ablation (shak-B2/shak-B2; + ; R91H05::GFP/R91H05::GFP) | n = 10 | 0.0019* | 0.0003* |
The individual, independent comparisons made with the KS2D2S test revealed whether there are differences in the distributions of response latency averages and the proportion of volume taken up by gap junction antibody in GF terminals, with significant differences shown by asterisks (*). The KS2D2S comparing response frequencies to the proportion of volume taken up by gap junction antibody in GF terminals shows the same significant differences as the latency test, except for UAS-FraE1354A (see results).