Intrinsic properties of epileptic L2/3 PNs separated based on AHP shape and latency
| Intrinsic property | fAHP neurons (n = 16, 12) | fAHP + ADP neurons (n = 35, 18) | mAHP neurons (n = 28, 13) | Statistical comparisons (p value) |
|---|---|---|---|---|
| Resting membrane potential (mV) | −70.29 ± 5.23 | −68.09 ± 7.25 | −65.73 ± 6.49 |
KW test p = 0.0577 |
| Input resistance (MΩ) | 135.10 ± 53.61 | 104.40 ± 45.38 | 137.90 ± 82.44 |
KW test p = 0.2531 |
| Voltage sag (%) | 4.19 ± 2.97 | 5.11 ± 2.61 | 6.51 ± 2.63 |
KW test p = 0.0194 |
| Membrane decay (ms) | 26.89 ± 6.02 | 26.16 ± 7.25 | 27.73 ± 7.29 |
ANOVA p = 0.7114 |
| AP threshold (mV) | −35.17 ± 6.58 | −37.46 ± 4.62 | −37.76 ± 5.68 |
KW test p = 0.6493 |
| AP amplitude (mV) | 78.92 ± 10.74 | 85.02 ± 7.40 | 84.98 ± 8.19 |
KW test p = 0.2345 |
| AP half-width (ms) | 1.26 ± 0.35 | 1.26 ± 0.36 | 1.40 ± 0.31 |
KW test p = 0.1375 |
| AHP magnitude (mV) | −16.42 ± 2.74 | −15.25 ± 3.56 | −15.14 ± 4.01 |
KW test p = 0.6069 |
| AHP latency (ms) | 4.35 ± 1.31 | 4.11 ± 1.36 | 20.84 ± 12.36 |
KW test p < 0.0001 |
| ΔAHP (mV) | −2.61 ± 3.06 | −2.49 ± 2.47 | −5.21 ± 2.91 |
KW test p = 0.0003 |
| AP broadening ratio | 1.26 ± 0.09 | 1.26 ± 0 0.11 | 1.27 ± 0.10 |
KW test p = 0.8880 |
| AP amplitude adaptation ratio | 0.93 ± 0.06 | 0.95 ± 0.03 | 0.90 ± 0.05 |
KW test p = 0.0026 |
| Initial instantaneous frequency (Hz) | 32.49 ± 15.72 | 33.53 ± 18.93 | 32.30 ± 13.25 |
KW test p = 0.9414 |
| Maximum firing rate (Hz) | 37.21 ± 13.94 | 35.09 ± 13.10 | 33.67 ± 12.03 |
KW test p = 0.6588 |
| Final instantaneous frequency (Hz) | 11.43 ± 4.71 | 9.78 ± 2.019 | 11.13 ± 4.36 |
KW test p = 0.4298 |
| Accommodation ratio | 0.38 ± 0.15 | 0.38 ± 0.15 | 0.39 ± 0.13 |
ANOVA p = 0.9230 |
| Max depolarization slope (dV/dT) | 207.80 ± 56.22 | 228.80 ± 39.37 | 206.70 ± 49.12 |
ANOVA p = 0.1265 |
| Max repolarization slope (dV/dT) | −50.44 ± 17.06 | −54.64 ± 10.75 | −44.74 ± 10.48 |
ANOVA p = 0.0072 |
Intrinsic properties of fAHP, fAHP-ADP, and mAHP epileptic L2/3 PNs.