Table 1.

Materials used for this study

Reagent or resourceSourceIdentifier
Antibodies
 Anti-α-Syn antibody (clone 42)BDRRID: AB_398107
 p-S129-α-Syn (clone EP1536Y)AbcamRRID: AB_869973
 p-S129-α-Syn (clone D1R1R)Cell Signaling TechnologyRRID: AB_279886
 Anti-vinculinSigma-AldrichRRID: AB_477629
 Anti-PSD95 (clone 7E3)Cell Signaling TechnologyRRID: AB_2721262
 Anti-GFAPNovus BiologicalsRRID: AB_829022
 Anti-Iba1Wako ChemicalsRRID: AB_839504
 Anti-THEMD MilliporeRRID: AB_390204
 Alexa Fluor 488 AffiniPure Donkey Anti-Mouse IgG (H + L)Jackson ImmunoResearch LaboratoriesRRID: AB_2340846
 Donkey Anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555Thermo Fisher ScientificRRID: AB_162543
 Donkey Anti-Goat IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488Thermo Fisher ScientificRRID: AB_2534102
Chemical
 1× protease inhibitor cocktailsGenDEPOTCatalog #P3100–100
 1× phosphatase inhibitor cocktailsGenDEPOTCatalog #P3200–020
 Laemmli sample bufferSigma-AldrichCatalog #S3401–10VL
 Amersham ECL Prime Western Blotting Detection ReagentCytivaCatalog #RPN2236
Kit
 VECTASTAIN(R) ELITE(R) ABC Anti-Rabbit IgG HRP Immunodetection KitVector LaboratoriesCatalog #PK-6101
 NativePage Sample kitThermo Fisher ScientificCatalog #BN2008
Experimental models: Organisms/strain
 Mouse: wild type (WT: C57BL/6J)Jackson LaboratoryCatalog #000664
 Mouse: SncaY39E KIThis paperN/A
 Mouse: SncaS129D KIThis paperN/A
CRISPR-Cas9 reagents
 Alt-R CRISPR-Cas9 tracrRNAIntegrated DNA TechnologiesCatalog #1072532
 Alt-R S.p. HiFi Cas9 Nuclease V3Integrated DNA TechnologiesCatalog #1081060
Oligonucleotides
 crRNA for SncaY39E: 5′-GGCAGCTGGAAAGACAAAAG-3′Integrated DNA TechnologiesN/A
 crRNA for SncaS129D: 5′-GGCTTATGAAATGCCTTCAG-3′Integrated DNA TechnologiesN/A
 ssODN for SncaY39E: 5′-TTCAAAGGCCAAGGAGGGAGTTGTGGCTGCTG CTGAGAAAACCAAGCAGGGTGTGGCAGAGGCAGCTGGAAAGACGAAG GAAGGCGTGCTGGAAGTAGGTAGGTAGTGACACTGTGACTAATGAATT GGGGTGGCTGGTGTGTGGTGTCTGATTCGTGTGCATCACAGCTTCTCA GAAGAGTGACAGCTGTGTG-3′Integrated DNA TechnologiesN/A
 ssODN for SncaS129D: 5′-GAGGAGGGGTACCCACAGGAAGGAATCCTGG AAGACATGCCTGTGGATCCTGGCAGCGAAGCCTACGAGATGCCGGAC GAAGTAAATGCCTGTATAAAGAAAACTAAGCAAAACACTTTAGGTGTTTA ATTTGGAACACATACCATCAAAACCCTGCCACTATCAGATCTCTCTCACA-3′Integrated DNA TechnologiesN/A
 Genotyping primer for SncaY39E and WT forward: anneals at 59° 5′-CCTCC CCTTCTGCAACTCTTCTTCTG-3′Sigma-AldrichN/A
 Genotyping primer for SncaY39E reverse: anneals at 59° 5′-CTTCCAGCACG CCTTCCTTCG-3′Sigma-AldrichN/A
 Genotyping primer for WT sequence of SncaY39E KI reverse: anneals at 59° 5′-GTCACTACCTACCTACATAGAGGACTCCCTCTTTTG-3′Sigma-AldrichN/A
 Genotyping primer for SncaS129D and WT forward: anneals at 63° 5′-GCCCT CATTATTCACCACACATGCACATAGTCCAC-3′Sigma-AldrichN/A
 Genotyping primer for WT sequence of SncaS129D KI reverse: anneals at 63° 5′-CTCTGAAGGCATTTCATAAGCCTCACT-3′Sigma-AldrichN/A
 Genotyping primer for SncaS129D reverse: anneals at 63° 5′-TTCGTCCGG CATCTCGTAGGCTTCGCT-3′Sigma-AldrichN/A
 Genomic sequencing primer for SncaY39E forward: 5′-CAACAATCAAT CAACACTGTGCC-3′Sigma-AldrichN/A
 Genomic sequencing primer for SncaY39E reverse: 5′-GTCTTTGATTCATAA GCATATTCTTGG-3′Sigma-AldrichN/A
 Genomic sequencing primer for SncaS129D forward: 5′-TCATTGAAAT GCTATGTGGGTTCTGTCTAC-3′Sigma-AldrichN/A
 Genomic sequencing primer for SncaS129D reverse: 5′-ACAAAATCCAGAT AACATTCATGACAGTAA-3′Sigma-AldrichN/A
 qPCR primer for mSnca forward: 5′-GTGACAACAGTGGCTGAGAAGAC-3′Sigma-AldrichN/A
 qPCR primer for mSnca reverse: 5′-GGTACCCCTCCTCACCCTTG-3′Sigma-AldrichN/A
 qPCR primer for mGapdh forward: 5′-AGGTCGGTGTGAACGGATTTG-3′Sigma-AldrichN/A
 qPCR primer for mGapdh reverse: 5′-TGTAGACCATGTAGTTGAGGTCA-3′Sigma-AldrichN/A