SPN dendritic morphology and soma area statistics
| Effects | Mean length/dendrite (µm) | Mean nodes/dendrite (µm) | Soma area (µm2) |
|---|---|---|---|
| Exposure (E) | F(1,232) = 1.030, p = 0.311 | F(1,232) = 0.007, p = 0.933 | F(1,232) = 1.030, p = 0.412 |
| Sex (S) | F(1,232) = 3.724, p = 0.055 | F(1,232) = 1.362, p = 0.244 | F(1,232) = 0.141, p = 0.707 |
| Postnatal age (P) | F(1,232) = 29.118, ***p < 0.001 | F(1,232) = 7.700, ***p < 0.001 | F(3,232) = 3.699,*p = 0.012 |
| E × S | F(1,232) = 1.162, p = 0.282 | F(1,232) = 7.159, **p = 0.008 | F(1,232) = 10.014,** p = 0.002 |
| E × P | F(3,232) = 1.503, p = 0.214 | F(3,232) = 0.901, p = 0.442 | F(3,232) = 1.503, p = 0.214 |
| P × S | F(3,232) = 11.774, ***p < 0.001 | F(3,232) = 2.335, p = 0.075 | F(3,232) = 2.890, *p = 0.036 |
| E × S × P | F(3,232) = 5.453, **p = 0.001 | F(3,232) = 1.037, p = 0.377 | F(3,232) = 1.540, p = 0.205 |
Statistical differences in mean length/dendrite, mean nodes/dendrite, and soma area were determined with three-way ANOVAs. Major effects: exposure (control chow vs prenatal ethanol exposure embryonic day 13.5–16.5), sex (male vs female), and postnatal day (P2, P4, P6, P8, P10, and P14), with interactions: E × S (exposure × sex), E × P (exposure × postnatal day), P × S (postnatal day × sex), and E × S × P (exposure × sex postnatal day). Statistics with p value ≤0.05 are bolded.