Key resources table
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus musculus) | C57BL/6 J | Charles River | Strain code 631 | |
| Genetic reagent (Rattus norvegicus) | Wistar (Crl:WI) | Charles River | Strain code: 003 | Used for preparation of glia feeder layer |
| Antibody | Anti-NPY (rabbit polyclonal) | Cell Signaling | 11,976 | 1:400 |
| Antibody | Anti-VAMP2 (mouse monoclonal) | Synaptic Systems | 104211 | 1:2,000 |
| Antibody | Anti-actin (mouse monoclonal) | Merck | MAB1501 | 1:1,000 |
| Antibody | Anti-MAP-2 (chicken polyclonal) | Abcam | ab5392 | 1:5,000 |
| Antibody | Anti-IA-2 (PTPRN) (mouse monoclonal) | Merck | MABS469 | 1:100 |
| Antibody | Anti-CHGB (rabbit polyclonal) | Synaptic Systems | 259103 | 1:500 |
| Antibody | Anti-BDNF (mouse monoclonal) | DSHB hybridoma product | BDNF #9 (supernatant)1 | 1:4 |
| Antibody | Anti-GAPDH (rabbit polyclonal) | Elabscience | E-AB-40337 | 1:2,000 |
| Recombinant DNA reagent | pLenti-Syn(pr)- TeNT-IRES- mCherry | Hoogstraaten et al. (2020) | - | |
| Recombinant DNA reagent | pLenti-Syn(pr)-pre-NPY-pHluorin | - | ||
| Sequence-based reagent | qPCR primers are Listed in Table 2 | |||
| Peptide, recombinant protein | 2.5% trypsin | Invitrogen | 15090046 | |
| Peptide, recombinant protein | Papain | Worthington Biochemical Corporation | LS003127 | |
| Peptide, recombinant protein | Poly-ʟ-ornithine | Sigma | P4957 | |
| Peptide, recombinant protein | Laminin | Sigma | L2020 | |
| Peptide, recombinant protein | Rat tail collagen | BD Biosciences | 354,236 | |
| Peptide, recombinant protein | Poly-d-lysine | Sigma | P6407 | |
| Commercial assay or kit | SensiFast cDNA Synthesis Kit | Meridian Bioscience | BIO-65054 | |
| Commercial assay or kit | SensiFast SYBR Lo-ROX Kit | Meridian Bioscience | BIO-94020 | |
| Software, algorithm | MATLAB | MathWorks | - | |
| Software, algorithm | Prism | GraphPad | - | |
| Software, algorithm | Fiji/ImageJ | NIH | - |