Forward and reverse primer sequences for target and reference genes. Asterisks indicate candidate reference genes.
| Gene | Primer sequence (5′-3′) | Size (bp) | Reference |
|---|---|---|---|
| timeless | F: TTAAGCCGACCGTAGTGCTG | 120 | - |
| R: CGTCTTCCGTCCATGTGTCT | |||
| Gαo | F: AAGCTGCTGCTCCTTGGTGCT | 147 | - |
| R: GGCGACGAGCGATTGAATAGTG | |||
| Gαq | F: CATTCACGGGTCGGGTTACA | 151 | - |
| R: CTCCGCCTTTTCTACGTTGG | |||
| Gαs | F: AGTCGACCATCGTGAAGCAG | 126 | - |
| R: TTGCACCGGTGATCGTAAGT | |||
| PLCβ1 | F: CGACACCATAAGCCAGTCCA | 115 | - |
| R: CGGTTTGCCGTCGGTTAAAT | |||
| PLCβ4 | F: GATATGGATCAGCCCCTCGC | 137 | - |
| R: AGTTCTACGCACCTGCATCC | |||
| RPS13* | F: GTCTTGCCCCTGACCTACCT | 173 | (Fenske et al., 2018) |
| R: TGGCAGCACACTCTTTGTCT | |||
| G3PDH* | F: CGATTAAGGAACTTGAGGACG | 232 | (Mészáros and Morton, 1996; Adamo et al., 2016) |
| R: ATAAGGAAGCGGATGCAAGG |