Comparison of NeuroART to other available software tools for real-time analysis of neuronal activity: CaImAn Online (Giovannucci et al., 2019), OnACID (Giovannucci et al., 2017), Vidrio ScanImage 2021, ClosedLoop (Zhang et al., 2018), Mitani et al. (Mitani and Komiyama, 2018), and ORCA (Sheng et al., 2022)
| NeuroART | CaImAn ONLINE (2019) | OnACID (2017) | ScanImage (2021) | ClosedLoop (2018) | Mitani et al. (2018) | ORCA (2022) | |
|---|---|---|---|---|---|---|---|
| Motion correction | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| Automated cell identification | ✓ | ✓ | ✓ | X | ✓ | X | ✓ |
| Fluorescence extraction | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| ΔF/F0 calculation | ✓ | ✓ | ✓ | X | ✓ | ✓ | ✓ |
| Functional network analysis | ✓ | X | X | X | X | X | X |
| Multi-timescale analysis (e.g., correlations, synchrony) | ✓ | X | X | X | X | X | X |
| Identification of stimulation targets | ✓ | ✓ | X | X | ✓ | X | ✓ |
| Real-time holographic optogenetic photostimulation | ✓ | X | X | X | X | X | X |
| Integration of sensory information | ✓ | X | X | X | X | X | ✓ |
| Works on multiple imaging and operating systems (OS) | ✓ | ✓ | X | ✓ | X | X | ✓ |