Colony breeding and survival in the three Atp1a3 het strains
| Atp1a3tm1Ling/+ | D801Y | D801N | ||||||
|---|---|---|---|---|---|---|---|---|
| Litter size and survival to weaning | ||||||||
| Strain | 6N | 6N | 6J | 6J | ||||
| Litters w pup counts | 66 | 66 | 14 on P0 | 32 at wean | ||||
| Pups/litter born | 7.24 | 7.05 | 6.2 | n.d. | ||||
| Pups/litter weaned | 7.03 | 6.61 | n.d. | 6.6 | ||||
| Survival | 98.0% | 93.8% | n.d. | (reduced) | ||||
| Proportion of mutants relative to wild type | ||||||||
| Litters genotyped | 105 litters at wean | 82 litters at wean | >50 litters on P0a | 32 at wean | ||||
| Genotype | WT | Het | WT | Het | WT | Het | WT | Het |
| Number of pups | 384 | 369 | 282 | 192 | 219 | 145 | 152 | 59 |
| Proportion | 0.51 | 0.49 | 0.595 | 0.405 | 0.602 | 0.398 | 0.72 | 0.28 |
| Fisher's exact test | p = 0.757 | p = 0.0041 | p = 0.0073 | p ≤ 0.0001 | ||||
| Hets as % of WT | 96% | 68% | 66% | 39% | ||||
| Binomial test | p = 0.610 | p ≤ 0.0001 | p ≤ 0.0001 | |||||
The genetic strain was C57BL/6N for Atp1a3tm1Ling/+ and Atp1a3+/D801Y mice and C57BL/6J for Atp1a3+/D801N. Litter size and survival to weaning was calculated for Atp1a3tm1Ling/+, D801Y, and D801N. Calculated proportions of surviving heterozygotes and WT at weaning, as well as at P0–P1 (D801N only) showed a non-Mendelian yield. The significance of WT and heterozygote proportions was determined by binomial test of observed to expected results with Fisher's exact test. Chi squared analyses gave qualitatively the same result but are regarded as approximations rather than exact tests. Runts and litters that failed due to lack of maternal care were not included because this is not uncommon in inexperienced C57BL/6 dams.
↵a The experimental aims for these cohorts were to use females, and so most male pups were not genotyped on P0. The data shown here for P0 were ∼80% female pups.