Table 1.

Details of probes for in situ HCR

Numbers	Probes	Target gene	HCR amplifier	Fluorophore
1	vglut1	Slc17a7	B1	Alexa 594
1	vglut1	Slc17a7	B4	Alexa 488
2	vglut2	Slc17a6	B1	Alexa 594
2	vglut2	Slc17a6	B4	Alexa 488
3	vglut2(set1)	Slc17a6_set1	B1	Alexa 594
4	vglut2(set2)	Slc17a6_set2	B4	Alexa 488
5	vglut3	Slc17a8	B4	Alexa 488
6	gad1	Gad1	B1	Alexa 594
7	gad2	Gad2	B4	Alexa 488
8	gad2(set1)	Gad2_set1	B1	Alexa 594
9	gad2(set2)	Gad2_set2	B4	Alexa 488
10	vgat	Slc32a1	B1	Alexa 594
11	parvalbumin	Pvalb	B4	Alexa 488
12	serotonin transporter	Slc6a4	B1	Alexa 594
13	gat	Slc6a1	B1	Alexa 594
14	enhanced green fluorescent protein	EGFP	B1	Alexa 594
15	choline acetyltransferase	ChAT	B4	Alexa 488
16	tyrosine hydroxylase	Th	B4	Alexa 488

The DNA probe set size, HCR amplifier, fluorophore, and for each target are shown.