Statistical table
| Figure | Structure | Type of test | Results |
|---|---|---|---|
| Fig. 1C | Normal | RM one-way ANOVA | PI+: F(2,52.2) = 42.7, p < 0.0001 |
| GCaMP-filled: F(2.58,67) = 18.8, p < 0.0001 | |||
| Fig. 1D (inset) | Normal | Unpaired t test (two-tailed) | t = 0.037, df = 32, p = 0.97 |
| Fig. 1F | Nonparametric | Spearman's correlation | Spearman's ρ = 0.66, p < 0.0001 |
| Extended Data Fig. 1-2C | Normal | Two-way RM ANOVA | Interaction: F(9,99) = 4.025, p = 0.0002 |
| Depth: F(9,99) = 16.8, p < 0.0001 | |||
| Treatment: F(1,11) = 12.3, p = 0.0049 | |||
| Extended Data Fig. 1-2D | Normal | Unpaired t test (two-tailed) | t = 3.51, df = 11, p = 0.005 |
| Extended Data Fig. 1-2E | Normal | Unpaired t test (two-tailed) | t = 2.76, df = 11, p = 0.02 |
| Extended Data Fig. 1-2F | Normal | Two-way RM ANOVA | Interaction: F(9,99) = 4.15, p = 0.0001 |
| Depth: F(9,99) = 6.18, p < 0.0001 | |||
| Treatment: F(1,11) = 12.3, p = 0.062 | |||
| Extended Data Fig. 1-2G | Normal | Two-way RM ANOVA | Interaction: F(9,90) = 2.18, p = 0.034 |
| Depth: F(9,90) = 14.1, p < 0.0001 | |||
| Treatment: F(1,10) = 0.041, p = 0.93 | |||
| Extended Data Fig. 1-3C | Normal | Two-way ANOVA | Interaction: F(6,87) = 0.3, p = 0.936 |
| Time point: F(3,87) = 9.2, p < 0.0001 | |||
| Layer: F(2,87) = 2.42, p = 0.095 | |||
| Extended Data Fig. 1-3D | Normal | Two-way ANOVA | Interaction: F(6,82) = 0.06, p = 0.99 |
| Time point: F(3,82) = 0.95, p = 0.42 | |||
| Layer: F(2,82) = 0.78, p = 0.45 | |||
| Extended Data Fig. 1-4A | Normal | Two-way RM ANOVA | Interaction: F(9,351) = 2.04, p = 0.034 |
| Depth: F(9,351) = 129.4, p < 0.0001 | |||
| Slice thickness: F(1,39) = 1.74, p = 0.125 | |||
| Extended Data Fig. 1-4B | Normal | Two-way RM ANOVA | Interaction: F(9,342) = 2.04, p = 0.0007 |
| Depth: F(9,342) = 52.51, p < 0.0001 | |||
| Slice thickness: F(1,38) = 1.81, p = 0.186 | |||
| Extended Data Fig. 1-4C | Normal | Two-way RM ANOVA | Interaction: F(9,216) = 0.08, p = 0.99 |
| Depth: F(9,216) = 91.3, p < 0.0001 | |||
| Age: F(1,24) = 0.36, p = 0.56 | |||
| Extended Data Fig. 1-4D | Normal | Two-way RM ANOVA | Interaction: F(9,306) = 0.87, p = 0.56 |
| Depth: F(9,306) = 54.16, p < 0.0001 | |||
| Age: F(1,34) = 10.49, p = 0.0027 | |||
| Fig. 2B | Normal | RM one-way ANOVA | PI+: F(2,52.2) = 42.7, p < 0.0001 |
| Neuropil: F(2,52.2) = 42.7, p < 0.0001 | |||
| Fig. 2C | Nonparametric | Spearman's correlation | Spearman's ρ =0.68, p < 0.0001 |
| Fig. 2E | Normal | Pearson's correlation | Pearson's r = 0.61, p < 0.0001 |
| Fig. 3B | Normal | Two-way RM ANOVA | Interaction: F(27,198) = 1.578, p = 0.043 |
| Depth: F(9,198) = 107.1, p < 0.0001 | |||
| Treatment: F(3,22) = 2.75, p = 0.07 | |||
| Fig. 3E | Normal | One-way ANOVA | PI+: F(3,22) = 3.06, p = 0.049 |
| Dunnett's multiple-comparisons test | |||
| 0–1 vs 1–2: p = 0.91, 0–1 vs 2–3: p = 0.99 | |||
| vs 3–5: p = 0.033 | |||
| GCaMP-filled: F(3,18) = 1.7, p = 0.2 | |||
| Dunnett's multiple-comparisons test | |||
| 0–1 vs 1–2: p = 0.97, 0–1 vs 2–3: | |||
| p = 0.96 | |||
| 0–1 vs 3–5: p = 0.13 | |||
| Fig. 5C | Nonparametric | Wilcoxon matched pairs test | W = 21, p = 0.031 |
| Fig. 5D | Nonparametric | Wilcoxon matched pairs test | W = 21, p = 0.031 |
| Fig. 5F | Nonparametric | Wilcoxon matched pairs test | W = 21, p = 0.031 |
| Fig. 5F | Nonparametric | Wilcoxon matched pairs test | W = 8, p = 0.375 |
| Fig. 6B | Normal | Two-way RM ANOVA | Interaction: F(9,369) = 3.32, p = 0.0006 |
| Depth: F(9,369) = 48.1, p < 0.0001 | |||
| Treatment: F(1,41) = 8.51, p = 0.0057 | |||
| Fig. 6B (inset) | Normal | Unpaired t test (two-tailed) | t = 3.19, df = 41, p = 0.002 |
| Fig. 6D | Normal | Two-way ANOVA | Interaction: F(3,34) = 0.97, p = 0.41 |
| Depth: F(3,34) = 4.57, p = 0.0091 | |||
| Treatment: F(1,34) = 15.4, p = 0.0004 | |||
| Sidak's multiple-comparisons test | |||
| <1 h: p = 0.67, 1–2 h: p = 0.21, 2–3 h: p = 0.023, 3–5 h: p = 0.011 | |||
| Fig. 6E | Normal | Two-way ANOVA | Interaction: F(3,33) = 1.4, p = 0.26 |
| Depth: F(3,33) = 0.71, p = 0.55 | |||
| Treatment: F(1,33) = 14.8, p = 0.0005 | |||
| Sidak's multiple-comparisons test | |||
| <1 h: p = 0.61, 1–2 h: p = 0.75, 2–3 h: p = 0.64, 3–5 h: p = 0.001 | |||
| Fig. 6F | Normal | Two-way RM ANOVA | Interaction: F(9,369) = 2.17, p = 0.023 |
| Depth: F(9,369) = 9.09, p < 0.0001 | |||
| Treatment: F(1,41) = 4.19, p = 0.047 | |||
| Fig. 6F (inset) | Nonparametric | Mann–Whitney test | U = 86, p = 0.002 |
| Fig. 6G | Normal | Two-way RM ANOVA | Interaction: F(9,369) = 1.46, p = 0.038 |
| Depth: F(9,369) = 23.7, p < 0.0001 | |||
| Treatment: F(1, 41) = 2.8, p = 0.01 | |||
| Fig. 6G (inset) | Normal | Unpaired t test (two-tailed) | t = 2.15, df = 41, p = 0.038 |
| Extended Data Fig. 6-1B (right) | Lognormal | One-way ANOVA (Tukey's post hoc test) | ANOVA, F(2,30) = 4.65. p = 0.017 |
| Tukey's multiple-comparisons test | |||
| aCSF vs MDL, p = 0.033 | |||
| aCSF vs Cal 2-1, p = 0.046 | |||
| MDL vs Cal 2-1, p = 0.910 | |||
| Extended Data Fig. 6-1B (right) | Lognormal | One-way ANOVA (Tukey's post hoc test) | ANOVA, F(2,31) = 5.79. p = 0.007 |
| Tukey's multiple-comparisons test | |||
| aCSF vs MDL, p = 0.005 | |||
| aCSF vs Cal 2-1, p = 0.188 | |||
| MDL vs Cal 2-1, p = 0.179 |