The ARRIVE guidelines 2.0 checklist: the essential 10
| Item | Item number | Recommendation | Reported in section | |
|---|---|---|---|---|
| Study design | 1 | a. | For each experiment, provide brief details of study design including: the groups being compared, including control groups. If no control group has been used, the rationale should be stated | Materials and Methods, Study design and Results |
| b. | The experimental unit (e.g., a single animal, litter, or cage of animals) | Materials and Methods, Study design and Table 5 | ||
| Sample size | 2 | a. | Specify the exact number of experimental units allocated to each group and the total number in each experiment. Also indicate the total number of animals used | Materials and Methods, Study design and Table 5 |
| b. | Explain how the sample size was decided. Provide details of any a priori sample size calculation, if done | Materials and Methods, Study design | ||
| Inclusion and exclusion criteria | 3 | a. | Describe any criteria used for including and excluding animals (or experimental units) during the experiment and data points during the analysis. Specify if these criteria were established a priori. If no criteria were set, state this explicitly | Materials and Methods, Field recordings |
| b. | For each experimental group, report any animals, experimental units, or data points not included in the analysis and explain why. If there were no exclusions, state so | Materials and Methods, Tables 5 and 6 | ||
| c. | For each analysis, report the exact value of n in each experimental group. | Materials and Methods, Study design and Table 5 | ||
| Randomization | 4 | a. | State whether randomization was used to allocate experimental units to control and treatment groups. If done, provide the method used to generate the randomization sequence | Materials and Methods, Study design |
| b. | Describe the strategy used to minimize potential confounders such as the order of treatments and measurements or animal/cage location. If confounders were not controlled, state this explicitly | Materials and Methods, Study design | ||
| Blinding | 5 | Describe who was aware of the group allocation at the different stages of the experiment (during the allocation, the conduct of the experiment, the outcome assessment, and the data analysis) | Materials and Methods, Study design | |
| Outcome measures | 6 | a. | Clearly define all outcome measures assessed (e.g., cell death, molecular markers, or behavioral changes) | Results; Materials and Methods, TEM and Table 5 |
| b. | For hypothesis-testing studies, specify the primary outcome measure, i.e., the outcome measure that was used to determine the sample size | Does not apply | ||
| Statistical methods | 7 | a. | Provide details of the statistical methods used for each analysis, including the software used | Materials and Methods, Statistics |
| b. | Describe any methods used to assess whether the data met the assumptions of the statistical approach and what was done if the assumptions were not met | Materials and Methods, Statistics | ||
| Experimental animals | 8 | a. | Provide species-appropriate details of the animals used, including species, strain and substrain, sex, age or developmental stage, and, if relevant, weight | Materials and Methods, Study design and key resources table |
| b. | Provide further relevant information on the provenance of animals, health/immune status, genetic modification status, genotype, and any previous procedures | Materials and Methods, Study design; Results; Discussion | ||
| Experimental procedures | 9 | a. | For each experimental group, including controls, describe the procedures in enough detail to allow others to replicate them, including: what was done, how it was done, and what was used | Materials and Methods |
| b. | When and how often | Materials and Methods, TEM, Table 5 | ||
| c. | Where (including detail of any acclimatization periods) | Materials and Methods, Acute slice preparation, Field recordings, and TEM | ||
| d. | Why (provide rationale for procedures) | Introduction; Results; Discussion; Materials and Methods | ||
| Results | 10 | a. | For each experiment conducted, including independent replications, report the summary/descriptive statistics for each experimental group, with a measure of variability where applicable (e.g., mean and SD or median and range) | Results, text and figure legends |
| b. | If applicable, the effect size with a confidence interval | Does not apply | ||
Adapted from Percie du Sert et al. (2020).