Summary of statistical information for all figures
| Figure | Comparison | Analysis | p value, F, or t value | N |
|---|---|---|---|---|
| 3A | Open field—total distance traveled by genotype | Two-way ANOVA with Tukey's multiple-comparisons test |
Sex: p = 0.6430; F(1,68) = 0.22 Genotype: p = 0.8755; F(1,68) = 0.025 Interaction: p = 0.1968; F(1,68) = 1.70 |
Male WT = 18 Female WT = 15 Male Tmem97 KO = 20 Female Tmem97 KO = 19 |
| 3B | Open field—time spent in center zone by genotype | Two-way ANOVA with Tukey's multiple-comparisons test |
Sex: ****p < 0.0001; F(1,68) = 35.04 Genotype: p = 0.2163; F(1,68) = 1.56 Interaction: p = 0.0918; F(1,68) = 2.92 Sex effect Male WT vs female WT: ****p < 0.0001 Male KO vs female KO: *p = 0.0160 |
Male WT = 18 Female WT = 15 Male Tmem97 KO = 20 Female Tmem97 KO = 19 |
| 3C | Open field—distance traveled in center zone by genotype | Two-way ANOVA with Tukey's multiple-comparisons test |
Sex: p = 0.6736; F(1,68) = 0.18 Genotype: p = 0.8806; F(1,68) = 0.023 Interaction: p = 0.4798; F(1,68) = 0.50 |
Male WT = 18 Female WT = 15 Male Tmem97 KO = 20 Female Tmem97 KO = 19 |
| 3D | Light/dark preference—number of entries to light side by genotype | Two-way ANOVA with Tukey's multiple-comparisons test |
Sex: p = 0.1888; F(1,68) = 1.76 Genotype: p = 0.0612; F(1,68) = 3.62 Interaction: p = 0.2503; F(1,68) = 1.34 |
Male WT = 18 Female WT = 15 Male Tmem97 KO = 20 Female Tmem97 KO = 19 |
| 3E | Light/dark preference—time spent in light side by genotype | Two-way ANOVA with Tukey's multiple-comparisons test |
Sex: p = 0.0589; F(1,68) = 3.69 Genotype: *p = 0.0205; F(1,68) = 5.63 Interaction: p = 0.1534; F(1,68) = 2.08 Genotype effect Male WT vs male KO: p = 0.9053 Female WT vs female KO: p = 0.0518 |
Male WT = 18 Female WT = 15 Male Tmem97 KO = 20 Female Tmem97 KO = 19 |
| 3F | Light/dark preference—average time spent per visit in light side by genotype | Two-way ANOVA with Tukey's multiple-comparisons test |
Sex: *p = 0.0137; F(1,68) = 6.40 Genotype: **p = 0.0097; F(1,68) = 7.08 Interaction: p = 0.1752; F(1,68) = 1.88 Sex effect Male WT vs female WT: *p = 0.0487 Male KO vs female KO: p = 0.8262 Genotype effect Male WT vs male KO: p = 0.7827 Female WT vs female KO: *p = 0.0358 |
Male WT = 18 Female WT = 15 Male Tmem97 KO = 20 Female Tmem97 KO = 19 |
| 3G | Light/dark preference—latency to first entry to light side by genotype | Two-way ANOVA with Tukey's multiple-comparisons test |
Sex: p = 0.7564; F(1,68) = 0.097 Genotype: p = 0.5405; F(1,68) = 0.38 Interaction: p = 0.2777; F(1,68) = 1.20 |
Male WT = 18 Female WT = 15 Male Tmem97 KO = 20 Female Tmem97 KO = 19 |
| 3H | Elevated plus maze—total distance traveled by genotype | Two-way ANOVA with Tukey's multiple-comparisons test |
Sex: *p = 0.0421; F(1,51) = 4.35 Genotype: p = 0.2287; F(1,51) = 1.48 Interaction: p = 0.0773; F(1,51) = 3.25 Sex effect Male WT vs female WT: p = 0.0635 Male KO vs female KO: p = 0.9964 |
Male WT = 15 Female WT = 9 Male Tmem97 KO = 16 Female Tmem97 KO = 15 |
| 3I | Elevated plus maze—number of entries in open arm by genotype | Two-way ANOVA with Tukey's multiple-comparisons test |
Sex: p = 0.4933; F(1,51) = 0.48 Genotype: p = 0.6114; F(1,51) = 0.26 Interaction: p = 0.1682; F(1,51) = 1.95 |
Male WT = 15 Female WT = 9 Male Tmem97 KO = 16 Female Tmem97 KO = 15 |
| 3J | Elevated plus maze—time spent in open arm by genotype | Two-way ANOVA with Tukey's multiple-comparisons test |
Sex: p = 0.8844; F(1,51) = 0.021 Genotype: p = 0.3175; F(1,51) = 1.02 Interaction: p = 0.6882; F(1,51) = 0.16 |
Male WT = 15 Female WT = 9 Male Tmem97 KO = 16 Female Tmem97 KO = 15 |
| 3K | Forced swim test—duration of immobility by genotype | Two-way ANOVA with Tukey's multiple-comparisons test |
Sex: p = 0.8739; F(1,68) = 0.025 Genotype: p = 0.3476; F(1,68) = 0.89 Interaction: p = 0.3526; F(1,68) = 0.88 |
Male WT = 18 Female WT = 15 Male Tmem97 KO = 20 Female Tmem97 KO = 19 |
| 3L | Tail suspension test—duration of immobility by genotype | Two-way ANOVA with Tukey's multiple-comparisons test |
Sex: p = 0.7254; F(1,68) = 0.12 Genotype: **p = 0.0013; F(1,68) = 11.24 Interaction: p = 0.1799; F(1,68) = 1.84 Genotype effect Male WT vs male KO: p = 0.4683 Female WT vs female KO: *p = 0.0100 |
Male WT = 18 Female WT = 15 Male Tmem97 KO = 20 Female Tmem97 KO = 19 |
| 4A | Z score on open field by genotype and sex | Two-way ANOVA with Tukey's multiple-comparisons test |
Sex: p = 0.6953; F(1,68) = 0.15 Genotype: p = 0.6787; F(1,68) = 0.17 Interaction: p = 0.6480; F(1,68) = 0.21 |
Male WT = 18 Female WT = 15 Male Tmem97 KO = 20 Female Tmem97 KO = 19 |
| 4B | Z score on light/dark preference | Two-way ANOVA with Tukey's multiple-comparisons test |
Sex: p = 0.0509; F(1,68) = 3.95 Genotype: *p = 0.0106; F(1,68) = 6.90 Interaction: p = 0.0944; F(1,68) = 0.094 Genotype effect Male WT vs male KO: p = 0.9045 Female WT vs female KO: *p = 0.0210 |
Male WT = 18 Female WT = 15 Male Tmem97 KO = 20 Female Tmem97 KO = 19 |
| 4C | Z score on elevated plus maze | Two-way ANOVA with Tukey's multiple-comparisons test |
Sex: p = 0.6683; F(1,51) = 0.19 Genotype: p = 0.4270; F(1,51) = 0.64 Interaction: p = 0.3565; F(1,51) = 0.87 |
Male WT = 15 Female WT = 9 Male Tmem97 KO = 16 Female Tmem97 KO = 15 |
| 4D | Z score on elevated zero maze | Two-way ANOVA with Tukey's multiple-comparisons test |
Sex: p = 0.1956; F(1,51) = 1.72 Genotype: p = 0.5834; F(1,51) = 0.30 Interaction: p = 0.5895; F(1,51) = 0.29 |
Male WT = 15 Female WT = 9 Male Tmem97 KO = 16 Female Tmem97 KO = 15 |
| 4E | Z score on forced swim test | Two-way ANOVA with Tukey's multiple-comparisons test |
Sex: p = 0.8752; F(1,68) = 0.025 Genotype: p = 0.3549; F(1,68) = 0.87 Interaction: p = 0.3454; F(1,68) = 0.9028 |
Male WT = 18 Female WT = 15 Male Tmem97 KO = 20 Female Tmem97 KO = 19 |
| 4F | Z score on tail suspension test | Two-way ANOVA with Tukey's multiple-comparisons test |
Sex: p = 0.7254; F(1,68) = 0.12 Genotype: **p = 0.0013; F(1,68) = 11.24 Interaction: p = 0.1799; F(1,68) = 1.84 Genotype effect Male WT vs male KO: p = 0.4683 Female WT vs female KO: *p = 0.0100 |
Male WT = 18 Female WT = 15 Male Tmem97 KO = 20 Female Tmem97 KO = 19 |
| 4G | Overall Z score on emotionality | Two-way ANOVA with Tukey's multiple-comparisons test |
Sex: p = 0.9888; F(1,68) = 0.00020 Genotype: ***p = 0.0008; F(1,68) = 12.37 Interaction: p = 0.8085; F(1,68) = 0.059 Genotype effect Male WT vs male KO: p = 0.0889 Female WT vs female KO: p = 0.0568 |
Male WT = 15–18 Female WT = 9–15 Male Tmem97 KO = 16–20 Female Tmem97 KO = 15–19 |
| 5B | Nestlet shredding—percent mass change | Two-way ANOVA with Tukey's multiple-comparisons test |
Sex: p = 0.6288; F(1,30) = 0.24 Genotype: p = 0.9455; F(1,30) = 0.0048 Interaction: p = 0.4405; F(1,30) = 0.61 |
Male WT = 11 Female WT = 5 Male Tmem97 KO = 12 Female Tmem97 KO = 6 |
| 5C | Marble burying—number of marbles buried | Two-way ANOVA with Tukey's multiple-comparisons test |
Sex: p = 0.0651; F(1,30) = 3.67 Genotype: p = 0.8778; F(1,30) = 0.024 Interaction: p = 0.1093; F(1,30) = 2.72 |
Male WT = 11 Female WT = 5 Male Tmem97 KO = 11 Female Tmem97 KO = 6 |
| 5D | Novel object recognition for time investigated—relative discrimination index | Two-way ANOVA with Tukey's multiple-comparisons test |
Sex: p = 0.9651; F(1,26) = 0.0019 Genotype: p = 0.5186; F(1,26) = 0.43 Interaction: p = 0.3282; F(1,26) = 0.99 |
Male WT = 11 Female WT = 5 Male Tmem97 KO = 8 Female Tmem97 KO = 6 |
| 5E | Novel object location for number of entries investigated—relative discrimination index | Two-way ANOVA with Tukey's multiple-comparisons test |
Sex: p = 0.4110; F(1,26) = 0.70 Genotype: p = 0.8019; F(1,26) = 0.064 Interaction: p = 0.6409; F(1,26) = 0.22 |
Male WT = 11 Female WT = 5 Male Tmem97 KO = 8 Female Tmem97 KO = 6 |
| 7A | Open field—total distance traveled at pre- and postsurgery | Three-way ANOVA with allowing different variances for genotype |
Treatment: p = 0.9523; F(1,64) = 0.0036 Sex: p = 0.1051; F(1,64) = 2.7 Genotype: p = 0.3333; F(1,64) = 0.95 Treatment × sex: p = 0.2051; F(1,64) = 1.64 Treatment × genotype: p = 0.4616; F(1,64) = 0.55 Sex × genotype: p = 0.2563; F(1,64) = 1.31 Treatment × sex × genotype: p = 0.8964; F(1,64) = 0.017 |
Male WT SNI = 9 Male WT sham = 9 Male KO SNI = 10 Male KO sham = 10 Female WT SNI = 9 Female WT sham = 6 Female KO SNI = 9 Female KO sham = 10 |
| 7B | Open field—distance traveled in center at pre- and postsurgery | Three-way ANOVA with allowing different variances for genotype |
Treatment: p = 0.4413; F(1,64) = 0.60 Sex: p = 0.6033; F(1,64) = 0.27 Genotype: p = 0.1211; F(1,64) = 2.47 Treatment × sex: p = 0.2860; F(1,64) = 1.16 Treatment × genotype: p = 0.8159; F(1,64) = 0.055 Sex × genotype: p = 0.1890; F(1,64) = 1.76 Treatment × sex × genotype: p = 0.4828; F(1,64) = 0.50 |
Male WT SNI = 9 Male WT sham = 9 Male KO SNI = 10 Male KO sham = 10 Female WT SNI = 9 Female WT sham = 6 Female KO SNI = 9 Female KO sham = 10 |
| 7C | Open field—time spent in center at pre- and postsurgery | Three-way ANOVA with allowing different variances for genotype |
Treatment: p = 0.6740; F(1,64) = 0.18 Sex: p = 0.2267; F(1,64) = 1.49 Genotype: p = 0.2688; F(1,64) = 1.24 Treatment × sex: p = 0.5807; F(1,64) = 0.31 Treatment × genotype: p = 0.2760; F(1,64) = 1.21 Sex × genotype: p = 0.2691; F(1,64) = 1.24 Treatment × sex × genotype: p = 0.1349; F(1,64) = 2.29 |
Male WT SNI = 9 Male WT sham = 9 Male KO SNI = 10 Male KO sham = 10 Female WT SNI = 9 Female WT sham = 6 Female KO SNI = 9 Female KO sham = 10 |
| 7D | Light/dark preference—number of entries to light side at pre- and postsurgery | Three-way ANOVA with allowing different variances for genotype and adjusted for Bonferroni’s method (nonsignificant interaction effect dropped for post hoc purpose) |
Treatment: p = 0.1393; F(1,65.4) = 2.24 Sex: *p = 0.0235; F(1,62) = 5.40 Genotype: p = 0.1323; F(1,62.1) = 2.33 Sex × treatment: *p = 0.0300; F(1,65.4) = 4.92 Genotype × sex: *p = 0.0227; F(1,62.1) = 5.46 Sex × treatment effect: adjusted p value Female SNI vs female sham: p = 0.9599 Female SNI vs male SNI: p = 0.9997 Female SNI vs male sham: *p = 0.0369 Female sham vs male SNI: p = 0.9350 Female sham vs male sham: *p = 0.0128 Male SNI vs male sham: *p = 0.0415 Genotype × sex effect: adjusted p value KO female vs KO male: p = 1.000 KO female vs WT female: p = 0.0502 KO female vs WT male: p = 0.9394 KO male vs WT female: *p = 0.0478 KO male vs WT male: p = 0.9329 WT female vs WT male: *p = 0.0177 Significant post hoc sex effects Sham WT male vs sham WT female: *p = 0.0011 SNI WT male vs SNI WT female: p = 0.3324 |
Male WT SNI = 9 Male WT sham = 9 Male KO SNI = 10 Male KO sham = 10 Female WT SNI = 9 Female WT sham = 6 Female KO SNI = 9 Female KO sham = 10 |
| 7E | Light/dark preference—time spent in light side at pre- and postsurgery | Three-way ANOVA with allowing different variances for genotype |
Treatment: p = 0.6966; F(1,64) = 0.15 Sex: p = 0.1308; F(1,64) = 2.34 Genotype: p = 0.4829; F(1,64) = 0.50 Treatment × sex: p = 0.3496; F(1,64) = 0.89 Treatment × genotype: p = 0.7381; F(1,64) = 0.11 Sex × genotype: p = 0.2320; F(1,64) = 1.46 Treatment × sex × genotype: p = 0.4074; F(1,64) = 0.70 |
Male WT SNI = 9 Male WT sham = 9 Male KO SNI = 10 Male KO sham = 10 Female WT SNI = 9 Female WT sham = 6 Female KO SNI = 9 Female KO sham = 10 |
| 7F | Light/dark preference—average time spent per visit in light side at pre- and postsurgery | Three-way ANOVA with allowing different variances for genotype |
Treatment: p = 0.6658; F(1,64) = 0.19 Sex: p = 0.8027; F(1,64) = 0.063 Genotype: p = 0.5007; F(1,64) = 0.46 Treatment × sex: p = 0.9823; F(1,64) = 0.00050 Treatment × genotype: p = 0.5690; F(1,64) = 0.33 Sex × genotype: p = 0.7305; F(1,64) = 0.12 Treatment × sex × genotype: p = 0.3753; F(1,64) = 0.80 |
Male WT SNI = 9 Male WT sham = 9 Male KO SNI = 10 Male KO sham = 10 Female WT SNI = 9 Female WT sham = 6 Female KO SNI = 9 Female KO sham = 10 |
| 7G | Light/dark preference—latency to first entry to light side at pre- and postsurgery | Three-way ANOVA with allowing different variances for genotype |
Treatment: p = 0.5038; F(1,64) = 0.45 Sex: p = 0.2468; F(1,64) = 1.37 Genotype: p = 0.5155; F(1,64) = 0.43 Treatment × sex: p = 0.3810; F(1,64) = 0.78 Treatment × genotype: p = 0.0935; F(1,64) = 2.90 Sex × genotype: p = 0.4062; F(1,64) = 0.70 Treatment × sex × genotype: p = 0.7113; F(1,64) = 0.14 |
Male WT SNI = 9 Male WT sham = 9 Male KO SNI = 10 Male KO sham = 10 Female WT SNI = 9 Female WT sham = 6 Female KO SNI = 9 Female KO sham = 10 |
| 7H | Time immobile in forced swim test at pre- and postsurgery | Three-way ANOVA with allowing different variances for genotype and adjusted for Bonferroni’s method (nonsignificant interaction effect dropped for post hoc purpose) |
Treatment: *p = 0.0221; F(1,66) = 5.50 Sex: p = 0.4848; F(1,66) = 0.49 Genotype: p = 0.1756; F(1,66) = 1.87 Sex × treatment: *p = 0.0440; F(1,66) = 4.21 Genotype × treatment: *p = 0.0155; F(1,66) = 6.17 Genotype × treatment effect: adjusted p value KO SNI vs KO sham: p = 0.9997 KO SNI vs WT SNI: *p = 0.0365 KO SNI vs WT sham: p = 0.9078 KO sham vs WT SNI: *p = 0.0421 KO sham vs WT sham: p = 0.8642 WT SNI vs WT sham: **p = 0.0021 Sex × treatment effect: adjusted p value Female SNI vs female sham: *p = 0.0186 Female SNI vs male SNI: p = 0.1972 Female SNI vs male sham: p = 0.1320 Female sham vs male SNI: p = 0.6580 Female sham vs Male sham: p = 0.7888 Male SNI vs male sham: p = 0.9953 Significant treatment post hoc effects SNI WT female vs sham WT female: ***p = 0.0003 |
Male WT SNI = 9 Male WT sham = 9 Male KO SNI = 10 Male KO sham = 10 Female WT SNI = 9 Female WT sham = 6 Female KO SNI = 9 Female KO sham = 10 |
| 7I | Time immobile in tail suspension test at pre- and postsurgery | Three-way ANOVA with allowing different variances for genotype and adjusted for Bonferroni’s method (nonsignificant interaction effect dropped for post hoc purpose) |
Treatment: p = 0.6647; F(1,65.3) = 0.19 Sex: *p = 0.0282; F(1,60.6) = 5.06 Genotype: **p = 0.0066; F(1,60.6) = 7.91 Genotype × sex: *p = 0.0321; F(1,60.6) = 4.81 Genotype × sex effect: adjusted p value KO female vs KO male: p = 1.0000 KO female vs WT female: **p = 0.0060 KO female vs WT male: p = 0.9763 KO male vs WT female: **p = 0.0048 KO male vs WT male: p = 0.9685 WT female vs WT male: *p = 0.0323 Significant sex post hoc effects SNI WT male vs SNI WT female: p = 0.3273 Sham WT male vs sham WT female: p = 0.3798 Significant genotype post hoc effects SNI WT female vs SNI KO female: p = 0.5202 Sham WT female vs sham KO female: p = 0.0652 |
Male WT SNI = 9 Male WT sham = 9 Male KO SNI = 10 Male KO sham = 10 Female WT SNI = 9 Female WT sham = 6 Female KO SNI = 9 Female KO sham = 10 |
| 8 | Mechanical hypersensitivity across time | Repeated-measures two-way ANOVA between time and group followed by Tukey's multiple-comparisons test at each time point |
Time: ****p < 0.0001; F(2.853,142.6) = 46.62 Group: ****p < 0.0001; F(3,50) = 16.62 Time × group: ****p < 0.0001; F(15,250) = 8.33 Baseline 1 WT SNI vs WT sham: p = 0.9594 WT SNI vs KO SNI: p = 0.9989 WT sham vs KO sham: p = 0.4629 KO SNI vs KO sham: p = 0.7256 Baseline 2 WT SNI vs WT sham: p = 0.9049 WT SNI vs KO SNI: p = 0.9661 WT sham vs KO sham: p = 0.2216 KO SNI vs KO sham: p = 0.9947 Post 7 d WT SNI vs WT sham: *p = 0.0418 WT SNI vs KO SNI: p = 0.9263 WT sham vs KO sham: p = 0.9921 KO SNI vs KO sham: **p = 0.0015 Post 14 d WT SNI vs WT sham: **p = 0.0022 WT SNI vs KO SNI: p = 0.7165 WT sham vs KO sham: p = 0.9520 KO SNI vs KO sham: ****p < 0.0001 Post 21 d WT SNI vs WT sham: ****p < 0.0001 WT SNI vs KO SNI: p > 0.9999 WT sham vs KO sham: p = 0.8188 KO SNI vs KO sham: ***p = 0.0003 Post 28 d WT SNI vs WT sham: ***p = 0.0002 WT SNI vs KO SNI: p = 0.9864 WT sham vs KO sham: p = 0.5849 KO SNI vs KO sham: ****p < 0.0001 |
WT sham = 11 WT SNI = 13 Tmem97 KO sham = 16 Tmem97 KO SNI = 14 |
| 3-1A | Elevated zero maze—total distance traveled by genotype | Two-way ANOVA with Tukey's multiple-comparisons test |
Sex: p = 0.2066; F(1,51) = 1.64 Genotype: p = 0.5897; F(1,51) = 0.29 Interaction: p = 0.1637; F(1,51) = 2.00 |
Male WT = 15 Female WT = 9 Male Tmem97 KO = 16 Female Tmem97 KO = 15 |
| 3-1B | Elevated zero maze—number of entries in open arm by genotype | Two-way ANOVA with Tukey's multiple-comparisons test |
Sex: p = 0.2613; F(1,51) = 1.29 Genotype: p = 0.6678; F(1,51) = 0.19 Interaction: p = 0.5986; F(1,51) = 0.28 |
Male WT = 15 Female WT = 9 Male Tmem97 KO = 16 Female Tmem97 KO = 15 |
| 3-1C | Elevated zero maze—time spent in open arm by genotype | Two-way ANOVA with Tukey's multiple-comparisons test |
Sex: p = 0.1635; F(1,51) = 2.00 Genotype: p = 0.5272; F(1,51) = 0.41 Interaction: p = 0.5967; F(1,51) = 0.28 |
Male WT = 15 Female WT = 9 Male Tmem97 KO = 16 Female Tmem97 KO = 15 |
| 8-1A | Time taken for walk initiation test | Repeated-measures mixed model ANOVA with allowing different variances for genotype and adjusted for Bonferroni’s method (nonsignificant interaction effect dropped for post hoc purpose) |
Time (pre/post): p = 0.2076; F(1,24) = 1.68 Genotype: p = 0.6009; F(1,23) = 0.28 Treatment: p = 0.2600; F(1,23) = 1.33 Time × treatment: *p = 0.0355; F(1,24) = 4.96 Time × treatment effect: adjusted p value Pre SNI vs post SNI: p = 0.1041 Post sham vs post SNI: p = 0.2189 Pre sham vs post SNI: p = 0.3998 Pre SNI vs post sham: p = 0.9593 Pre sham vs pre SNI: p = 0.9996 Pre sham vs post sham: p = 0.9013 |
WT sham = 7 WT SNI = 8 Tmem97 KO sham = 7 Tmem97 KO SNI = 4 |
| 8-1B | Time taken to come down to the bottom of the pole | Repeated-measures mixed model ANOVA with allowing different variances for genotype |
Time (pre/post): p = 0.1908; F(1,22) = 1.82 Genotype: p = 0.8413; F(1,22) = 0.04 Treatment: p = 0.8458; F(1,22) = 0.04 Time × genotype: p = 0.8469; F(1,22) = 0.04 Time × treatment: p = 0.1271; F(1,22) = 2.51 Genotype × treatment: p = 0.3423; F(1,22) = 0.94 Time × genotype × treatment: p = 0.2302; F(1,22) = 1.52 |
WT sham = 7 WT SNI = 8 Tmem97 KO sham = 7 Tmem97 KO SNI = 4 |
| 8-1C | Time taken to make the first turn on the pole | Repeated-measures mixed model ANOVA with allowing different variances for genotype |
Time (pre/post): p = 0.5788; F(1,22) = 0.32 Genotype: p = 0.9752; F(1,22) = 0.00 Treatment: p = 0.4152; F(1,22) = 0.69 Time × genotype: p = 0.3913; F(1,22) = 0.76 Time × treatment: p = 0.4790; F(1,22) = 0.52 Genotype × treatment: p = 0.9425; F(1,22) = 0.01 Time × genotype × treatment: p = 0.9324; F(1,22) = 0.01 |
WT sham = 7 WT SNI = 8 Tmem97 KO sham = 7 Tmem97 KO SNI = 4 |
| 8-1D | Time stayed on the ledge without falling | Repeated-measures mixed model ANOVA with allowing different variances for genotype |
Time (pre/post): p = 0.1869; F(1,22) = 1.86 Genotype: p = 0.1869; F(1,22) = 1.86 Treatment: p = 0.1869; F(1,22) = 1.86 Time × genotype: p = 0.1869; F(1,22) = 1.86 Time × treatment: p = 0.1869; F(1,22) = 1.86 Genotype × treatment: p = 0.1869; F(1,22) = 1.86 Time × genotype × treatment: p = 0.1869; F(1,22) = 1.86 |
WT sham = 7 WT SNI = 8 Tmem97 KO sham = 7 Tmem97 KO SNI = 4 |
| 8-1E | Time stayed on the platform without falling | Repeated-measures Mixed Model ANOVA with allowing different variances for genotype |
Time (pre/post): p = 0.3829; F(1,22) = 0.79 Genotype: p = 0.3829; F(1,22) = 0.79 Treatment: p = 0.3829; F(1,22) = 0.79 Time × genotype: p = 0.3829; F(1,22) = 0.79 Time × treatment: p = 0.3829; F(1,22) = 0.79 Genotype × treatment: p = 0.3829; F(1,22) = 0.79 Time × genotype × treatment: p = 0.3829; F(1,22) = 0.79 |
WT sham = 7 WT SNI = 8 Tmem97 KO sham = 7 Tmem97 KO SNI = 4 |
| 8-1F | Latency time to go up to the top of the 60° inclined wire mesh | Repeated-measures mixed model ANOVA with allowing different variances for genotype |
Time (pre/post): p = 0.4457; F(1,22) = 0.60 Genotype: p = 0.2325; F(1,22) = 1.51 Treatment: p = 0.6187; F(1,22) = 0.25 Time × genotype: p = 0.8701; F(1,22) = 0.027 Time × treatment: p = 0.5581; F(1,22) = 0.35 Genotype × treatment: p = 0.4541; F(1,22) = 0.58 Time × genotype × treatment: p = 0.3706; F(1,22) = 0.84 |
WT sham = 7 WT SNI = 8 Tmem97 KO sham = 7 Tmem97 KO SNI = 4 |
| 8-1G | Latency time to go down to the bottom of the 60° inclined wire mesh | Repeated-measures mixed model ANOVA with allowing different variances for genotype |
Time (pre/post): ****p < 0.0001; F(1,22) = 33.51 Genotype: p = 0.1658; F(1,22) = 2.05 Treatment: p = 0.8178; F(1,22) = 0.05 Time × genotype: p = 0.1658; F(1,22) = 2.05 Time × treatment: p = 0.8178; F(1,22) = 0.05 Genotype × treatment: p = 0.7309; F(1,22) = 0.12 Time × genotype × treatment: p = 0.7309; F(1,22) = 0.12 |
WT sham = 7 WT SNI = 8 Tmem97 KO sham = 7 Tmem97 KO SNI = 4 |
| 8-1H | Latency time to go up to the top of the 90° inclined wire mesh | Repeated-measures mixed model ANOVA with allowing different variances for genotype |
Time (pre/post): ***p = 0.0006; F(1,22) = 15.81 Genotype: p = 0.3877; F(1,22) = 0.78 Treatment: p = 0.4373; F(1,22) = 0.63 Time × genotype: p = 0.2603; F(1,22) = 1.34 Time × treatment: p = 0.1912; F(1,22) = 1.82 Genotype × treatment: p = 0.2726; F(1,22) = 1.27 Time × genotype × treatment: p = 0.4752; F(1,22) = 0.53 |
WT sham = 7 WT SNI = 8 Tmem97 KO sham = 7 Tmem97 KO SNI = 4 |
| 8-1I | Latency time to go down to the bottom of the 90° inclined wire mesh | Repeated-measures mixed model ANOVA with allowing different variances for genotype |
Time (pre/post): **p = 0.0053; F(1,22) = 9.59 Genotype: p = 0.0704; F(1,22) = 3.62 Treatment: p = 0.4517; F(1,22) = 0.59 Time × genotype: p = 0.0704; F(1,22) = 3.62 Time × treatment: p = 0.4517; F(1,22) =0.59 Genotype × treatment: p = 0.6716; F(1,22) = 0.18 Time × genotype × treatment: p = 0.6716; F(1,22) = 0.18 |
WT sham = 7 WT SNI = 8 Tmem97 KO sham = 7 Tmem97 KO SNI = 4 |
| 8-1J | Latency time to fall from inversed wire mesh | Repeated-measures mixed model ANOVA with allowing different variances for genotype |
Time (pre/post): **p = 0.0086; F(1,22) = 8.32 Genotype: p = 0.6758; F(1,22) = 0.18 Treatment: p = 0.2138; F(1,22) = 1.64 Time × genotype: p = 0.3852; F(1,22) = 0.78 Time × treatment: p = 0.5172; F(1,22) = 0.43 Genotype × treatment: p = 0.9073; F(1,22) = 0.01 Time × genotype × treatment: p = 0.5435; F(1,22) = 0.38 |
WT sham = 7 WT SNI = 8 Tmem97 KO sham = 7 Tmem97 KO SNI = 4 |
Statistical significance was determined at the level of p < 0.05. Asterisks denoting p values include *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. All data are presented as the mean ± standard error of the mean (SEM).