Statistical analysis with significance
| Analysis/figure | Test | Statistical value | Pairwise comparisons | p value |
|---|---|---|---|---|
| Number of SARS-CoV-2 cells (Fig. 2) | Two-way ANOVA + Tukey's multiple-comparison test |
Mean diff. −851.7 95.00% CI of diff. −1,601 to −102.5 | PC - LOT vs L1 | 0.0202 |
|
Mean diff. −804.3 95.00% CI of diff. −1,554 to −55.13 | PC - L1 vs L2 | 0.0309 | ||
|
Mean diff. −964.2 95.00% CI of diff. −1,920 to −8.442 | TuS - L1 vs L2 | 0.0476 | ||
| Fractal analysis (cell morphology; Fig. 3) | Welch's unpaired t test | t = 3.746, df = 12.58 | Control vs infected | 0.0026 |
| Macrophages counts in the OE (Fig. 4) | Multiple unpaired t tests | t = 2.917, df = 4.000 | IBA1+ in septum | 0.0434 |
| Microglia counts in the OB (Fig. 5) | Multiple unpaired t tests | t = 5.104, df = 4.000 | IBA1+ total (average) | 0.006963 |
| t = 3.147, df = 4.000 | IBA1+/Clec7A+ total (average) | 0.034601 | ||
| t = 3.992, df = 4.000 | IBA1+ in ONL | 0.016240 | ||
| t = 3.955, df = 4.000 | IBA1+ in GL | 0.016746 | ||
| t = 5.812, df = 4.000 | IBA1+/Clec7A+ in GL | 0.004362 | ||
| t = 3.541, df = 4.000 | IBA1+ in EPL | 0.023985 | ||
| t = 3.989, df = 4.000 | IBA1+ in GCL | 0.016275 | ||
| t = 2.878, df = 4.000 | IBA1+ in RMS | 0.045123 | ||
| Microglia counts in PC and TuS (Fig. 6) | Multiple unpaired t tests | t = 12.46, df = 4.000 | IBA1+ in PC - total (average) | 0.000239 |
| t = 8.485, df = 4.000 | IBA1+ in PC - LOT | 0.001058 | ||
| t = 10.85, df = 4.000 | IBA1+ in PC - L1 | 0.000409 | ||
| t = 19.35, df = 4.000 | IBA1+ in PC - L2 | 0.000042 | ||
| t = 9.483, df = 4.000 | IBA1+ in PC - L3 | 0.000690 | ||
| t = 6.632, df = 4.000 | IBA1+ in TuS - total (average) | 0.002681 | ||
| t = 4.255, df = 4.000 | IBA1+ in TuS - L2 | 0.013110 | ||
| t = 4.834, df = 4.000 | IBA1+ in TuS - L3 | 0.008437 | ||
| Average pixel intensity CNPase (Fig. 7) | Multiple unpaired t tests | t = 6.042, df = 4.000 | CNPase pixels LOT | 0.003785 |
| t = 5.229, df = 4.000 | CNPase pixels AC | 0.006389 |