Table 1

Experimental list

FiguresPurposeExperimentsNumber of mice
Figure 1A–FTo assess the effect of stroke and EA treatment on the synaptic activityElectrophysiological recording in slicesGAD67-GFP mice: Sham group (N = 7), Stroke group (N = 5), and Stroke + EA group (N = 5)
Figure 1G,HTo validated whether the tonic inhibition was changedElectrophysiological recording in slices9/3*
Figure 2A–GTo explore the expressions of PNNs in different conditions and show the injection sites of PNNs degradation and test the efficacy of injection of P-nase or ChABC(1) Injection of P-nase or ChABC
(2) Immunofluorescence		P-nase-injected group (N = 2)
ChABC-injected group (N = 2)
27/3*
Figure 3A–CTo explore the role of PNNs in the regulation of neuronal activity in the stroke or EA treatment(1) Injection of P-nase or ChABC
(2) Immunofluorescence		18/3*
Figure 4A–ETo explore the role of PNNs in the regulation of neuronal activity in the stroke or EA treatment(1) Injection of P-nase or ChABC
(2) In vivo electrophysiological recording		24/4*
Figure 5A–FTo record the sEPSCs and sIPSCs(1) Injection of P-nase
(2) Electrophysiological recording in slices		Sham group (N = 6), Stroke group (N = 4), and Stroke + EA group (N = 3)
Figure 5A–FTo record the sEPSCs and sIPSCs(1) Injection of ChABC
(2) Electrophysiological recording in slices		Sham group (N = 6), Stroke group (N = 3) and Stroke + EA group (N = 3)
Figure 6A,BTo validate the function of PNNs in the EA-mediated improvement of swallowing function(1) Injection of P-nase or ChABC
(2) Electromyography recording in the mylohyoid in vivo		36/6*
Total = 160 mice, GAD67-GFP = 17 mice, C57 = 143 mice