Primers for qPCR analysis
| Gene | Sequence | Tm (°C) | Amplicon size (bp) | Primer efficiency |
|---|---|---|---|---|
| actb2 | Fw: CCAAACCCAAGTTCAGCCATGG | 62.25 | 118 | 99.1% |
| Rv: TGGATGGGAAGACAGCACGG | 62.18 | |||
| th | Fw: TTCAGCCATACCAAGACCAG | 61.6 | 147 | 109.4% |
| Rv: CTTCTATGCTGTCCGTGTACG | 61.2 | |||
| ddc | Fw: ACCCTGATGTGGAACCGGGA | 63.05 | 137 | 131.7% |
| Rv: GTATGGGCTGTGCCAGTGGG | 63.18 | |||
| slc18a2 (vmat) | Fw: CGGAGGCTGATTCTGTTGATCG | 61.37 | 120 | 106.4% |
| Rv: CATCTGAGCAGCCTCGTCGT | 62.28 | |||
| slc6a3 (dat) | Fw: AAACACAACGTGGCCCTGGA | 62.56 | 108 | 90.9% |
| Rv: GACTGCCCACACCGAAGAGC | 63.39 | |||
| drd1b | Fw: TGACAAGGTCTGTGGGAGTACA | 63.4 | 147 | 104.2% |
| Rv: ACGTGAATCGGAGCAACTGG | 66.3 | |||
| drd2a | Fw: CCTCCATTGTGTCCTTCTACG | 62.3 | 123 | 93.7% |
| Rv: TGTCTGTAACTGGGCATGTG | 61.2 | |||
| drd2b | Fw: GCTTTCATTCGCCATTTCCTG | 66.5 | 142 | 93.6% |
| Rv: GGTGATAATGAAGGGCACGTAG | 63.3 | |||
| drd3 | Fw: TCTTTGTGACCCTGGATGTG | 62.5 | 140 | 92.1% |
| Rv: CATGACTGAAACCCTTTTGCG | 65.4 |
List of genes and associated designed primers for qPCR analysis. Gene names are italicized: actin, β2 (actb2); tyrosine hydroxylase (th); DOPA decarboxylase (ddc), Synaptic vesicle monoamine transporter (vmat), dopamine transporter (dat), dopamine receptor type Ib (drd1b), dopamine receptor type 2a (drd2a), dopamine receptor type 3 (drd3).