Table 5

Few significant changes in hippocampal gene expression following treatment with TIMP2 or TIMP2-hIgG4

Modality	Gene	TIMP2	TIMP2- hIgG4	Kruskal–Wallis test statistics
Neuronal	Dcx	0.6790	0.4208	H(2) = 4.646, p = 0.0980
	Tubb3	0.8189	0.1729	H(2) = 2.939, p = 0.2300
	Syn1	*0.0294	0.9810	H(2) = 10.31, p = 0.0058
	Dlg4	0.0616	0.6662	H(2) = 4.667, p = 0.0970
	Gria1	0.4569	0.8582	H(2) = 3.828, p = 0.1475
	Grin2a	>0.9999	0.1800	H(2) = 3.138, p = 0.2082
	Slc2a1	0.1370	0.9185	H(2) = 6.614, p = 0.0366
	Gad1	0.3957	>0.9999	H(2) = 3.438, p = 0.1793
Immediate early genes	Cfos	*0.0173	**0.0087	H(2) = 9.773, p = 0.0075
	Creb1	0.2678	>0.9999	H(2) = 3.817, p = 0.1483
	Egr1	0.7935	0.1351	H(2) = 3.342, p = 0.1880
Microglia	Cd68	0.6144	>0.9999	H(2) = 1.509, p = 0.4702
	Iba1	>0.9999	>0.9999	H(2) = 0.2837, p = 0.8678
	Cd11b	0.5904	0.9339	H(2) = 3.016, p = 0.2213
Inflammatory	Il1a	0.7900	>0.9999	H(2) = 2.050, p = 0.3589
	Il1b	>0.9999	0.9233	H(2) = 0.9005, p = 0.6375
	Il6	0.5032	>0.9999	H(2) = 1.516, p = 0.4686
	Ccl11	0.1789	>0.9999	H(2) = 3.675, p = 0.1592
	Nfkb	0.9367	0.1776	H(2) = 2.903, p = 0.2342
	Tnfa	0.3888	>0.9999	H(2) = 1.687, p = 0.4301
Astrocytes	Gfap	>0.9999	>0.9999	H(2) = 0.7345, p = 0.6926
	Aqp4	0.2516	0.3938	H(2) = 7.558, p = 0.0228
	Ggta1	>0.9999	0.1737	H(2) = 5.146, p = 0.0763

Kruskal–Wallis test statistics and p-values of post hoc Dunn’s multiple comparisons tests for comparisons between average gene expression relative to Gapdh measured by Taqman or SYBR qPCR from bulk hippocampal tissue from Vehicle versus the TIMP2 and TIMP2-hIgG4 treatment groups. n = 9–13 mice per group.