The alanine insertion into TIMP2 prevented MMP inhibitory activity at biologically relevant concentrations
| IC50 (nm) | MMP2 | MMP3 | MMP9 |
|---|---|---|---|
| TIMP2 | 1.65 | 7.37 | 2.00 |
| Ala-TIMP2 | 2321 | 1689 | 127.7 |
| TIMP2-hIgG4 | 1.82 | 9.70 | 1.46e−5 |
The IC50 values (nm) of TIMP2, Ala-TIMP2, and TIMP2-hIgG4 inhibitory activity on MMP2, MMP3, and MMP9.