Table 2

Specific methods used for each antibody for immunohistology

Primary antibodyConcentrationBufferSecondary antibodyConcentrationBuffer
22524-1-AP, Thermo Fisher Scientific (lot #000025322)1:100, 1:2002% NGS, 0.01% Triton X-100Anti-rabbit IRdye 680
(lot #C51104-05)		1:20002% NGS, 0.01% Triton X-100
AB2231, Sigma-Aldrich (lot #NG1900345)1:100, 1:2002% NGS, 0.01% Triton X-100Anti-rabbit IRdye 680
(lot #C51104-05)		1:20002% NGS, 0.01% Triton X-100
SC-32258, Santa Cruz (lot #F0722; G1217)1:100, 1:2002% NGS, 0.01% Triton X-100Anti-rat IRdye 680
(lot #21321)		1:20002% NGS, 0.01% Triton X-100
SC-32259, Santa Cruz (lot #l1021; l2717)1:100, 1:2002% NGS, 0.01% Triton X-100Anti-rat IRdye 680
(lot #21321)		1:20002% NGS, 0.01% Triton X-100
SC-58517, Santa Cruz (lot #C2216)1:100, 1:2002% NGS, 0.01% Triton X-100Anti-rat IRdye 680
(lot #21321)		1:20002% NGS, 0.01% Triton X-100
MAB369, Millipore Sigma 
(lot #3188610)		1:100, 1:2002% NGS, 0.01% Triton X-100Anti-rat IRdye 680
(lot #21321)		1:20002% NGS, 0.01% Triton X-100
ZRB1525, Millipore Sigma (lot #Q3480251;
Q3451217)		1:100, 1:2002% NGS, 0.01% Triton X-100; 2% NGSAnti-rabbit IRdye 680, anti-rabbit Alexa Fluor 568 dye
(lot #C51104-05;927620)		1:2000; 1:5002% NGS, 0.01% Triton X-100, 2% NGS
MA5-24796, Thermo Fisher Scientific (lot #XF3606356)1:100, 1:2002% NGS, 0.01% Triton X-100Anti-mouse IRdye 680, anti-mouse Alexa Fluor 488
(lot #C50721-05;1110070)		1:2000; 1:5002% NGS, 0.01% Triton X-100, 2% NGS
431-DATC, Phospho-
Solutions (lot #ajo1016y)		1:100, 1:2002% NGS, 0.01% Triton X-100Anti-rabbit IRdye 680
(lot #C51104-05)		1:20002% NGS, 0.01% Triton X-100
D6944, Millipore Sigma (lot #000018709;
069M4849V)		1:2002% NGS, 0.01% Triton X-100; 2% NGS (FM)Anti-rabbit IRdye 680, anti-rabbit Alexa Fluor 568 dye
(lot #C51104-05;927620)		1:2000; 1:5002% NGS, 0.01% Triton X-100, 2% NGS
Anti-TH Antibody Pel Freez (P40101-150)1:500 (rat TH verification)2% NGS, 0.01% Triton X-100Anti-rabbit IRdye 680
(lot #C51104-05)		1:20002% NGS, 0.01% Triton X-100