Specific methods used for each antibody for western blotting
| Antibody | Gel | Protein | Concentration | Transfer | Blocking | Antibody buffer |
|---|---|---|---|---|---|---|
| MAB369; Millipore Sigma (lot #3188610) | Novex 4–20% Tris-Glycine | Mouse striatum total lysate | 1:750 | 90v-90m-RT | LI-COR for 2 h at room temperature | LI-COR |
| 600-401-C75; Rockland (lot #27999) | Novex 4–20% Tris-Glycine | Mouse striatum total lysate | 1:1000 | 90v-90m-RT | LI-COR for 2 h at room temperature | LI-COR |
| AB2231; Sigma-Aldrich (lot #NG1900345) | Novex 4–20% Tris-Glycine | Mouse striatum total lysate | 1:1000 | 90v-90m-RT | LI-COR for 2 h at room temperature | LI-COR |
| SC-1433; Santa Cruz (lot #B1413) | Novex 10% Tris-Glycine | Mouse striatum total lysate | 1:750 | 90v-90m-RT | LI-COR for 2 h at room temperature | LI-COR |
| SC-32259; Santa Cruz (lot #l1021; l2717) | Novex 10% Tris-Glycine | Mouse striatum total lysate | 1:750 | 90v-90m-RT | LI-COR for 2 h at room temperature | LI-COR |
| SC-32258; Santa Cruz (lot #F0722; G1217) | Novex 10% Tris-Glycine | Mouse striatum total lysate | 1:750 | 90v-90m-RT | LI-COR for 2 h at room temperature | LI-COR |
| SC-58517; Santa Cruz (lot #C2216) | Novex 10% Tris-Glycine | Mouse striatum total lysate | 1:750 | 90v-90m-RT | LI-COR for 2 h at room temperature | LI-COR |
| 431-DATC; Phospho-Solutions (lot #ajo1016y) | Homemade 10% Tris-Glycin acrylamide | Mouse striatum total lysate or cell total lysate | 1:1000 | 90v-90m-RT | LI-COR for 2 h at room temperature | LI-COR |
| 434-DATEL2; Phospho-Solutions (lot #jh417g) | Novex 10% Tris-Glycine | Mouse striatum total lysate | 1:1000 | 90v-90m-RT | LI-COR for 2 h at room temperature | LI-COR |
| 22524-1-AP; Thermo Fischer Scientific (lot #000025322) | Homemade 10% Tris-Glycin acrylamide | Mouse striatum total lysate or cell total lysate | 1:1000 | 90v-90m-RT | LI-COR for 1.5 h at room temperature | LI-COR |
| GTX30992; Gene Tex (lot #821800340) | Homemade 10% Tris-Glycin acrylamide | Mouse striatum total lysate | 1:500 | 90v-90m-RT | LI-COR for 1.5 h at room temperature | LI-COR |
| D6944; Millipore Sigma (lot #000018709; 069M4849V) | Homemade 10% Tris-Glycin acrylamide | Mouse striatum total lysate | 1:500 | 90v-90m-RT | LI-COR for 1.5 h at room temperature | 5% BSA in TBS+ 0.05% Tween 20 |
| MA5-24796; Thermo Fisher Scientific (lot #XF3606356) | Novex 10% Tris-Glycine | Mouse striatum membrane preparation | 1:250 | 22v-ON-4 | 5% BSA in TBS for 1 h at room temperature | 5% BSA In TBS+ 0.05% Tween 20 |
| ZRB1525; Millipore Sigma (lot #Q3480251; Q3451217) | Novex 10% Tris-Glycine | Mouse striatum membrane preparation | 1:100 | 22v-ON-4 | 5% BSA in TBS for 1 h at room temperature | 5% BSA In TBS+ 0.05% Tween 20 |