Table 2

Primers used for qPCR analyses

GeneAccession no.Amplified regionForward primer (5′–>3′)Reverse primer (5′–>3′)
GapdhBC085315735–843GCATGGCCTTCCGTGTTCCTAGATGCCTGCTTCACCACCTTCT
Etv1L10426221–392GCAAGTGCCTTACGTGGTCACAGGTACCTGAGCTTCAGCAAG
1320–1517CGTTGGGGCATTCAGAAGAACTTCCATGTCCGTCTTCAGCAG
Tas1r1AF3370401651–1795ACAATCAGGTGCCTGTGTCAGTGGCAGGTGTGAAGCTCACTC
Tas1r2AF3370411284–1446TCGTCTATCCATGGCAGCTACTAGGAGGCGATGCTTTGGAAG
Tas1r3AF337039481–652ACCTTCAACGGCACCCTTCAGCAGGTGAAGTCATCTGGATGC
Gnat3BC147839530–679GACTTAGACAGACTCACAGCCCTCTGATCTCTGGCCACCTAC
Plcb2NM_1775681731–1857CCTGGAGGTGACAGCTTATGAGAGCTCCGTGAAGGAAGAGAC
Trpm5AF2286813320–3491GGAAAACGGCACACAGAGTGGCCACAGTTCTGAGAGCTTGAG
Calhm1LC270870535–665TTGCGCTGCATCTCTCAGGCATAGTGGGACCAGTACTTGCTC
Calhm3LC270871453–571GCAAGTGCAGCTCTTCCTAGCTCCAGCCGATGGCCTGTGACA
Tas2r104BC148229488–635ACATCCTGGCTGATCCACCTGATTGCATCTGCCTGCTGTGTC
Tas2r105BC125541160–318TGACTGGCTTCCTTCTCATCGTCAGGTGATTCACAGTCATCC
Tas2r118BC104401298–411GCTGTCCTCTACTGTGTCAAGAGAAGCTATCAGAGCACCCAG
Tas2r126NM_207028511–678AAGGAGTGGAACAGAAGGCTGGTTGGGGTCTTGCAAGCTGTG
Scnn1aNM_0113242107–2259GTCTGTGAAGTCCCAGGATTGACCATCGTGACAGAGGGAGAC
Pkd2l1NM_1814221715–1921TGCCATCGACAATGCCAACAGCGCAGCCTTAGTAGGGTCTTG
Entpd2NM_009849395–560CATGCGCCTACTCAACCTGACGTTCTCCAGCAGGTAGTTGGC