Detailed experimental protocol for neurobehavioral analysis
| Control (n = 11) | BT(OF) (n = 11) | BT(EE) (n = 11) | |
|---|---|---|---|
| Day 1: habituation | Rats were placed in an empty NOR apparatus and were allowed to freely explore the arena for 5 min. | ||
| Day 2: training | Two similar shaped objects were placed in NOR apparatus and rats were allowed to explore the objects for 5 min. Single trial training was provided to all groups. | ||
| No novelty exposure was provided | 15 min after training, rats were subjected to 5-min open field task as novelty | 15 min after training, rats were subjected to 5-min environment enrichment as novelty | |
| Day 3: test | One object was replaced with a novel object in NOR apparatus and rats were tested for 5 min for NOR long-term memory | ||
Experimental protocol for studying the effect of different types of novelty exposure on memory consolidation process. Animals were randomly divided into three experimental groups: control, BT(OF), and BT(EE) with n = 11 for each group. All groups were subjected to NOR as the learning task, while OF and EE were two different types of novelties used to induce the BT phenomenon. Control group was not given novelty exposure. Complete neurobehavioral experiment consisted of three days protocol following 5 min of habituation, single trial training (followed by novelty for two groups) and testing session.