Rod outer segment suction recordings analysis parameters
Idark (pA) | SfDA (× 10−3 phot−1 μm2) | I1/2 (phot μm−2) | Tp (ms) | Tint (ms) | τrec (ms) | N | |
---|---|---|---|---|---|---|---|
wild type | 13 ± 1 | 8 ± 1 | 89 ± 14 | 215 ± 12 | 941 ± 60 | 273 ± 24 | 14 cells |
RhoF45L KI | 12 ± 1 | 10 ± 1 | 76 ± 15 | 227 ± 10 | 965 ± 39 | 357 ± 61 | 13 cells |
p value | 0.06 | 0.52 | 0.55 | 0.43 | 0.74 | 0.22 |
Idark, saturated response amplitude measured at the plateau.
SfDA, dark-adapted sensitivity.
I1/2, intensity required to produce half of the saturated response.
Tp, time to peak of a dim flash response.
Tint, integration time of the response.
τrec, recovery time constant during response shut off.