Statistical analyses of AP firing after 0, 4, or 8 weeks of UCMS in IL layer II/III PV+ neurons from male and female mice
| Test | UCMS | Sex | UCMS × sex | Stimulus | Stimulus × UCMS | Stimulus × sex | Stimulus × sex × UCMS |
|---|---|---|---|---|---|---|---|
| AP firing: sex as a factor | |||||||
| Three-way ANOVA with repeated measures | F(2,87) = 7.71, p < 0.001 | F(1,87) = 2.20, p = 0.14 | F(2,87) = 2.68, p = 0.074 | F(1.84,159.6) = 41.47, p < 0.001 | F(3.67,159.6) = 9.63, p < 0.001 | F(1.84,159.6) = 0.65, p = 0.51 | F(3.67,159.6) =1.42, p = 0.23 |
| AUC analysis, two-way ANOVA | F(2,87) = 6.99, p = 0.0015 | F(1,87) = 2.41, p = 0.12 | F(2,87) = 2.64, p = 0.077 | ||||
| AP Firing by sex | |||||||
| Males, two-way ANOVA | F(2,39) = 10.86, p = 0.0002 | F(1.75,68.2) = 12.34, p < 0.0001 | F(14,273) = 3.88, p < 0.0001 | ||||
| Females, two-way ANOVA | F(2,48) = 1.27, p = 0.29 | F(1.91,91.9) = 35.67, p < 0.0001 | F(14,336) = 8.89, p < 0.0001 | ||||
| AP height at threshold | |||||||
| Three-way ANOVA with repeated measures | F(2,87) = 4.99, p = 0.009 | F(1,87) = 0.93, p = 0.34 | F(2,87) = 0.008, p = 0.99 | F(1.05,91.4) = 25.05, p < 0.001 | F(2.10,91.4) = 0.18, p = 0.85 | F(1.05,91.4) = 1.71, p = 0.19 | F(2.10,91.4) = 0.51, p = 0.61 |
| AP height at threshold by sex | |||||||
| Males, two-way ANOVA | F(2,39) = 2.11, p = 0.14 | F(1.12,43.8) = 14.15, p = 0.0003 | F(4,78) = 1.27, p = 0.29 | ||||
| Females, two-way ANOVA | F(2,48) = 3.03, p = 0.058 | F(1.03,49.5) = 15.30, p = 0.0002 | F(4,96) = 0.089, p = 0.99 | ||||
| AP height at suprathreshold | |||||||
| Three-way ANOVA with repeated measures | F(2,87) = 20.04, p < 0.001 | F(1,89) = 0.04, p = 0.85 | F(2,89) = 0.97, p = 0.38 | F(1.16,101.0) = 254.23, p < 0.001 | F(2.32,101.0) = 28.87, p < 0.001 | F(1.16,101.0) = 1.19, p = 0.29 | F(2.32,101.1) = 1.53, p = 0.22 |
| AP height at suprathreshold by sex | |||||||
| Males, two-way ANOVA | F(2,39) = 7.51, p = 0.0017 | F(1.05,40.8) = 141.1, p < 0.0001 | F(4,78) = 13.28, p < 0.0001 | ||||
| Females, two-way ANOVA | F(2,48) = 20.92, p < 0.0001 | F(1.21,58.1) = 131.1, p < 0.0001 | F(4,96) = 18.12, p < 0.0001 |
Greenhouse–Geisser corrected for sphericity violations, as needed, indicated by noninteger degrees of freedom. Stimulus indicates intensity of current injected for AP firing and spike number for AP height.