Property | UCMS | Sex | UCMS × sex | Effect |
---|---|---|---|---|
Resting membrane potential | F(2,87) = 6.10 p = 0.0033 | F(1,87) = 2.10, p = 0.15 | F(2,87) = 2.37, p = 0.10 | UCMS8 > control |
Input resistance | F(2,87) = 4.12 p = 0.020 | F(1,87) = 0.12, p = 0.73 | F(2,87) = 0.53, p = 0.59 | UCMS4 > control |
Total APs | F(2,87) = 6.06, p = 0.0034 | F(1,87) = 2.86, p = 0.094 | F(2,87) = 1.61 p = 0.21 | UCMS8 > control |
First evoked AP amplitude (threshold) | F(2,87) = 6.60 p = 0.0021 | F(1,87) = 1.11 p = 0.29 | F(2,87) = 0.16 p = 0.85 | Control > UCMS8 |
Third evoked AP amplitude (threshold) | F(2,87) = 6.63 p = 0.0021 | F(1,87) = 0.22 p = 0.64 | F(2,87) = 0.079 p = 0.92 | Control > UCMS8 |
Third evoked AP amplitude (suprathreshold) | F(2,87) = 39.32 p < 0.0001 | F(1,87) = 0.61 p = 0.44 | F(2,87) = 1.57 p = 0.21 | Control > UCMS4 > UCMS8 |
Maximum frequency at rheobase | F(2,87) = 2.86 p = 0.063 | F(1,87) = 2.08, p = 0.15 | F(2,87) = 2.29 p = 0.11 | |
Maximum frequency | F(2,87) = 7.74 p = 0.0008 | F(1,87) = 23.76, p = 0.056 | F(2,87) = 3.97 p = 0.023 | Control, UCMS4 > UCMS8 |
AP threshold | F(2,87) = 2.51 p = 0.087 | F(1,87) = 1.68, p = 0.20 | F(2,87) = 1.38, p = 0.26 | |
AP half-width* | F(2,87) = 1.29 p = 0.28 | F(1,87) = 0.52, p = 0.47 | F(2,87) = 3.48 p = 0.035 | |
Females only, Kruskal–Wallis test | H(3) = 0.052, p = 0.97 | |||
Males only, Kruskal–Wallis test | H(3) = 11.21, p = 0.0037 | UCMS8 > control |
These tests were followed up by separate analyses by sex via Kruskal–Wallis tests. Comparisons in the Effect column are post hoc Tukey’s tests for parametric and Dunn’s multiple-comparisons tests for nonparametric statistics.
*Two-way ANOVA results are shown as non-normally distributed data, which could not be adjusted by mathematical transformation.