Primary and secondary antibodies used in the study
| Primary antibodies | ||||
|---|---|---|---|---|
| Anti | Host | Isotype | Dilution | Company (catalog #) |
| GFP | Chicken | Polyclonal | 1:500 | Abcam (Ab13970) |
| GFAP | Mouse | Monoclonal IgG1 | 1:400 | Sigma-Aldrich (G3893) |
| CD31 | Mouse | Monoclonal IgG1 | 1:50 | Abcam (ab9498) |
| Iba1 | Rabbit | Polyclonal | 1:200 | Wako (019–19741) |
| CC3 | Rabbit | Polyclonal | 1:400 | Cell Signaling (9661) |
| MERTK | Rat | Monoclonal IgG2a | 1:100 | Invitrogen (14-5751-82) |
| CLEC7A | Rat | Monoclonal IgG2a | 1:30 | Invivogen (mabg-mdect) |
| CD68 | Rat | Monoclonal IgG2a | 1:200 | Biorad (FA-11) |
| DCX | Guinea pig | Polyclonal | 1:400 | Sigma-Aldrich (AB2253) |
| Secondary antibodies | ||||
| Anti | Host | Isotype | Dilution | Company |
| Chicken | Donkey | A488 | 1:400 | The Jackson Laboratory |
| Chicken | Goat | Biotin | 1:400 | Abcam |
| Mouse | Goat | A647 | 1:400 | Molecular Probes |
| Rabbit | Donkey | A488 | 1:400 | Molecular Probes |
| Rabbit | Donkey | A555 | 1:400 | Molecular Probes |
| Rat | Donkey | A647 | 1:400 | The Jackson Laboratory |
| Guinea pig | Goat | A555 | 1:400 | Molecular Probes |