Table 6

Mean ± SEM and two-way ANOVA parameters for GnRH neurons hyperpolarized between −90 and −95 mV (Fig. 4)

Current (pA) needed to hyperpolarize the cell to −90 to −95 mV
VEH 3 weeks	PNA 3 weeks	VEH adult	PNA adult
−37.3 ± 1.95	−35.0 ± 1.78	−31.0 ± 2.67	−31.5 ± 1.73

Steady-state to sag-peak membrane potential difference
VEH 3 weeks	PNA 3 weeks	VEH adult	PNA adult
0.2 ± 0.12	0.04 ± 0.20	−0.01 ± 0.15	0.3 ± 0.26

Repolarization to basal membrane potential difference
VEH 3 weeks	PNA 3 weeks	VEH adult	PNA adult
0.3 ± 0.26	0.3 ± 0.48	−0.1 ± 0.26	0.5 ± 0.32

Two-way ANOVA
Property	Age	Treatment		Interaction
Current (pA) needed to hyperpolarizethe cell to −90 to −95 mV	Diff, −4.867 [Cl, −8.960, −0.7745] F_(1,41) = 5.768; p = 0.0209	Diff, −0.8671 [Cl, −4.960, 3.225] F_(1,41) = 0.1831; p = 0.6710		Diff, −2.811 [Cl, −11.0, 21.915.374 F_(1,41) = 0.4811; p = 0.4918
	Bonferroni	3-week VEH vs 3-week PNA	3-week VEH vs adult VEH	3-week PNA vs adult PNA	Adult VEH vs adult PNA
		p > 0.9999	p = 0.2398	p > 0.9999	p > 0.9999
Steady-state to sag-peak potential difference	Diff, −0.04333 [Cl, −0.4615, 0.3748] F_(1,41) = 0.04380; p = 0.8353	Diff, −0.09561 [Cl, −0.5138, 0.00928503226] F_(1,41) = 0.2132; p = 0.6467		Diff, 0.4902 [Cl, −0.3461, 1.327] F_(1,41) = 1.401; p = 0.2433
Repolarization to basal potential difference	Diff, 0.09892 [Cl, −0.5909, 0.7887] F_(1,41) = 0.08386; p = 0.7736	Diff, −0.3164 [Cl, −1.006, 0.3735] F_(1,41) = 0.8579; p = 0.3597		Diff, 0.7403 [Cl, −0.6393, 2.120] F_(1,41) = 1.174; p = 0.2848

Bold indicates p < 0.05.