Detailed description of statistical analyses used in this study
| Figure | Parameter | Sample size | Statistical test | Significance level |
|---|---|---|---|---|
| 1G | tdTomato+ neurons in P28 spinal cord | N = 3 mice per group | Ordinary one-way ANOVA with Tukey’s multiple comparisons test | F(3,8) = 90.22, p < 0.0001 C vs T: p = 0.0003 C vs L: p = 0.0001 C vs S: p < 0.0001 T vs S: p < 0.0001 L vs S: p = 0.0002 |
| 1H | tdTomato+ gray matter astrocytes in P28 spinal cord | N = 3 mice per group | Ordinary one-way ANOVA with Tukey’s multiple comparisons test | F(3,8) = 81.54, p < 0.0001 C vs T: p = 0.0004 C vs L: p = 0.0004 C vs S: p < 0.0001 T vs S: p = 0.0001 L vs S: p = 0.0002 |
| 1I | tdTomato+ white matter astrocytes in P28 spinal cord | N = 3 mice per group | Ordinary one-way ANOVA with Tukey’s multiple comparisons test | F(3,8) = 26.84, p = 0.0002 C vs T: p = 0.0486 C vs L: p = 0.0206 C vs S: p < 0.0001 T vs S: p = 0.0022 L vs S: p = 0.0045 |
| 1J | tdTomato+ neurons in P28 spinal cord (dorsal vs ventral) | N = 3 mice per group | Two-way ANOVA with Sidak’s multiple comparisons test | F(1,16) = 133.7, p < 0.0001 (source of variation: dorsal vs ventral) T dorsal vs T ventral: p < 0.0001 L dorsal vs L ventral: p < 0.0001 S dorsal vs S ventral: p < 0.0001 |
| 1K | tdTomato+ gray matter astrocytes in P28 spinal cord (dorsal vs ventral) | N = 3 mice per group | Two-way ANOVA with Sidak’s multiple comparisons test | F(1,16) = 51.32, p < 0.0001 (source of variation: dorsal vs ventral) T dorsal vs T ventral: p = 0.0004 L dorsal vs L ventral: p = 0.0294 S dorsal vs S ventral: p < 0.0001 |
| 1L | tdTomato+ white matter astrocytes in P28 spinal cord (dorsal vs ventral) | N = 2–3 mice per group) | Two-way ANOVA with Sidak’s multiple comparisons test | F(1,14) = 25.83, p = 0.0002 (source of variation: dorsal vs ventral) S dorsal vs S ventral: p < 0.0001 |
| 1M | Percent of all Sox9+ cells that express tdTomato in P28 spinal cord | N = 3 mice per group | Ordinary one-way ANOVA with Tukey’s multiple comparisons test | F(3,8) = 242.9, p < 0.0001 C vs L: p = 0.0055 C vs S: p < 0.0001 T vs L: p = 0.0289 T vs S: p < 0.0001 L vs S: p < 0.0001 |
| 2C | tdTomato+ Schwann cells at NMJs in P28 mice | N = 3–4 per group (between 110 and 344 NMJs quantified per individual muscle) | Ordinary one-way ANOVA with Bonferroni’s multiple comparisons test | F(5,14) = 3.392, p = 0.0324 TS vs SOL: p = 0.0306 TS vs EDL: p = 0.0539 |
| 7D | tdTomato+ neurons in vitro | N = 12 wells per group | Unpaired t test | t = 25.15, df = 22 p < 0.0001 |
| 7E | tdTomato+ Sox9+ cells in vitro | N = 6 wells per group | Unpaired t test | t = 8.772, df = 10 p < 0.0001 |
| 7F | tdTomato+ Olig2+ cells in vitro | N = 6 wells per group | Unpaired t test | t = 8.287, df = 10 p < 0.0001 |
| 8C | Cluster size in vitro + drug treatment | N = 6 wells per group (between 153 and 319 clusters quantified per well) | Ordinary one-way ANOVA with Dunnett’s multiple comparisons test | F(4,1096) = 363.7, p < 0.0001 VEH vs CHIR: p < 0.0001 VEH vs FGF: p < 0.0001 VEH vs FGF+GDF: p < 0.0001 |
| 8E | Percent neurons in vitro + drug treatment | N = 6 wells per group | Ordinary one-way ANOVA with Dunnett’s multiple comparisons test | F(4,25) = 8.967, p = 0.0001 VEH vs FGF: p = 0.0003 |
| 8F | Percent astrocytes in vitro + drug treatment | N = 6 wells per group | Ordinary one-way ANOVA with Dunnett’s multiple comparisons test | F(4,25) = 36.21, p < 0.0001 VEH vs FGF: p < 0.0001 VEH vs FGF+GDF: p = 0.0027 |
| 8G | Percent tdTomato+ cells in vitro + drug treatment | N = 6 wells per group | Ordinary one-way ANOVA with Dunnett’s multiple comparisons test | F(4,25) = 2.502, p = 0.0680 No significant differences |
| 8H | Percent tdTomato+ neurons in vitro + drug treatment | N = 6 wells per group | Ordinary one-way ANOVA with Dunnett’s multiple comparisons test | F(4,25) = 3.596, p = 0.0190 No significant differences |
| 8I | Percent tdTomato+ astrocytes in vitro + drug treatment | N = 6 wells per group | Ordinary one-way ANOVA with Dunnett’s multiple comparisons test | F(4,25) = 6.630, p = 0.0009 VEH vs CHIR: p = 0.0244 VEH vs FGF+GDF: p = 0.0002 |