Seeding densities immediately after seeding and live count taken just before seeding
| High cell density | Low cell density | |
|---|---|---|
| Total number of neurons | 75,000 | 37,500 |
| Total number of astrocytes | 75,000 | 37,500 |
| Seeding cell density (cells/mm2) | 5882 | 2941 |
| Astrocyte live count before seeding | 70% | 72% |
| Neuron live count before seeding | 62.5 | 69% |
Only live cells were counted in total number of astrocytes and neurons, and thus the seeding cell density and total number of neurons and astrocytes reflects the live cells as included by trypan blue staining.