Off-Target primers: primers for amplifying and sequencing candidate off-target sites
| Off-target site | Guide | Chromosome | Gene | Mismatch | Forward primer | Reverse primer | Sequencing primer |
|---|---|---|---|---|---|---|---|
| 1 | Upstream | 9 | Trank1 | 3 | AGGGCCCTTAGCATTTTTGC | AGCCAGCTGCAAGGAAACTC | GGATGTCTCCCTCTATGCAGTG |
| 2 | Upstream | 7 | 3 | GTACTGTCTTGGTGAGGGATTG | CCCAACAGAAGGCAGAGTAAG | CTCATACCATACCGTGCAG | |
| 3 | Upstream | 1 | 3 | CAGAGGCAGGTGGATTTCTAAG | CGGTTCTAGGATTGCTGTTCTC | TTCTCCATCACCATGCTGTG | |
| 4 | Upstream | 18 | 3 | AGTTGCATCTCACAGTTCCTATC | CTATGGGCCGGCTTCTATTATG | GTAAGCTGATCTCTCCGATC | |
| 5 | Upstream | 12 | 3 | GAATACAGAGCTGAGGGAATGG | GGAGAACATGAGAGCTGGATATG | CAGTAGTAGAGCCATCCTTAC | |
| 6 | Upstream | 17 | 3 | GATTATCAGCAGGGCTAGGATG | AGAGAGCATTGTGGGAATGAG | CCTTTAGCTCTGGGAGGTTTC | |
| 7 | Upstream | 5 | 3 | GGTTCAGTTGCTTCCCAGTT | CCCGAAAGCTTGATCGAAGAG | CTGAGGATCTAGAAGCACTG | |
| 8 | Upstream | 7 | 3 | TTCTGTACGGTTGGCTTCTTC | GGTGAGTCATCTCAGCAATCTC | ACTTCAGGAGTCTTCTCTC | |
| 9 | Upstream | 9 | Mcam | 3 | TGAGGGTAAGGAGAGGGTAAG | GTACCATAGGACTTGAGGAATGG | GTCGCTTGCTCTTACACAG |
| 10 | Upstream | 4 | Ctnnbip1 | 4 | TGCCTCAGCCGGAAATAAG | ACACAGACACACAGACACATAG | CTAGGGTCTCAAGCCTTC |
| 11 | Downstream | 7 | 3 | CCACCTTACACTAGCCATGAAC | CAAGGGCAGGATTGGAAGATAC | AGAGACACAGAGGGACAAG | |
| 12 | Downstream | 7 | 3 | CCACCTTACACTAGCCATGAAC | CAAGGGCAGGATTGGAAGATAC | AGAGACACAGAGGGACAAG |