Secondary antibodies and dilutions used in the study
Antibody and fluorochrome | Conjugated fluorophore | Dilution | Source | Code no. | RRID |
---|---|---|---|---|---|
Donkey anti-rat IgG | Biotin | 1:250 | Jackson ImmunoResearch | 712–065-153 | AB_2315779 |
Donkey anti-guinea pig IgG | Biotin | 1:250 | Jackson ImmunoResearch | 706–065-148 | AB_2340451 |
Donkey anti-mouse IgG | Alexa Fluor 488 | 1:250 | Jackson ImmunoResearch | 715–545-151 | AB_2341099 |
Donkey anti-rabbit IgG | Alexa Fluor 488 | 1:250 | Thermo Fisher Scientific | A21206 | AB_2535792 |
Donkey anti-rabbit IgG | Rhodamine red | 1:250 | Jackson ImmunoResearch | 711–295-152 | AB_2340613 |
Donkey anti-goat IgG | Rhodamine red | 1:500 | Jackson ImmunoResearch | 705–295-147 | AB_2340423 |
Streptavidin | Alexa Fluor 647 | 1:250 | Jackson ImmunoResearch | 016–600-084 | AB_2341101 |