Table 3

ANOVA results of Western blots of hippocampus

Protein	Interaction	Main effect	F_(df,error) value	p value
pAKT Ser473	Treatment × Genotype		F_(1,19) = 0.190	0.668
		Treatment	F_(1,19) = 0.097	0.759
		Genotype	F_(1,19) = 1.820	0.193
pAKT Thr308	Treatment × Genotype		F_(1,19) = 6.766	0.018^*
		Treatment	F_(1,19) = 30.807	<0.001^*
		Genotype	F_(1,19) = 5.334	0.032^*
pAMPK	Treatment × Genotype		F_(1,19) = 1.856	0.189
		Treatment	F_(1,19) = 0.018	0.895
		Genotype	F_(1,19) = 0.019	0.893
pCREB	Treatment × Genotype		F_(1,19) = 0.170	0.684
		Treatment	F_(1,19) = 0.195	0.663
		Genotype	F_(1,19) = 0.540	0.471
peIF2α	Treatment × Genotype		F_(1,19) = 0.177	0.678
		Treatment	F_(1,19) = 0.837	0.372
		Genotype	F_(1,19) = 0.651	0.430
pERK	Treatment × Genotype × Band		F_(1,19) = 0.155	0.699
	Treatment × Band		F_(1,19) = 0.069	0.796
	Genotype × Band		F_(1,19) = 0.328	0.574
	Treatment × Genotype		F_(1,19) = 0.652	0.429
		Treatment	F_(1,19) = 0.001	0.971
		Genotype	F_(1,19) = 0.248	0.624
		Band	F_(1,19) = 127.210	<0.001^*
pGSK3α/β	Treatment × Genotype × Band		F_(1,19) = 0.661	0.426
	Treatment × Band		F_(1,19) = 0.227	0.639
	Genotype × Band		F_(1,19) = 1.259	0.276
	Treatment × Genotype		F_(1,19) = 0.692	0.416
		Treatment	F_(1,19) = 0.388	0.541
		Genotype	F_(1,19) = 0.262	0.614
		Band	F_(1,19) = 265.256	<0.001^*
pmTOR	Treatment × Genotype		F_(1,19) = 0.032	0.861
		Treatment	F_(1,19) = 4.789	0.041^*
		Genotype	F_(1,19) = 0.135	0.717
p-p70 S6K Thr389	Treatment × Genotype × Band		F_(1,19) = 0.191	0.667
	Treatment × Band		F_(1,19) = 0.096	0.760
	Genotype × Band		F_(1,19) = 1.127	0.302
	Treatment × Genotype		F_(1,19) = 0.020	0.889
		Treatment	F_(1,19) = 0.046	0.832
		Genotype	F_(1,19) = 0.003	0.955
		Band	F_(1,19) = 325.069	<0.001^*
p-p70 S6K Thr421/Ser424	Treatment × Genotype × Band		F_(1,19) = 0.231	0.636
	Treatment × Band		F_(1,19) = 0.463	0.504
	Genotype × Band		F_(1,19) = 0.075	0.788
	Treatment × Genotype		F_(1,19) <0.001	0.998
		Treatment	F_(1,19) = 0.762	0.394
		Genotype	F_(1,19) = 0.942	0.344
		Band	F_(1,19) = 140.106	<0.001^*
pS6	Treatment × Genotype × Site		F_(1,19) = 1.343	0.261
	Treatment × Site		F_(1,19) = 0.406	0.532
	Genotype × Site		F_(1,19) = 0.506	0.485
	Treatment × Genotype		F_(1,19) = 0.337	0.569
		Treatment	F_(1,19) = 6.396	0.020^*
		Genotype	F_(1,19) = 0.092	0.765
		Site	F_(1,19) = 38.962	<0.001^*

Values are the mean ± SEM for the number of mice indicated in parentheses. Whereas experiments were completed in eight WT and five Tsc2^Mjg^+/− mice, the number of mice analyzed depended on the quality of the autoradiograms at the level of each ROI. Bonferroni-corrected post hoc tests showed that Tsc2^Mjg^+/− mice had statistically significantly lower rCPS in all brain regions analyzed.