CRISPR knock-ins
| Gene | Protein | Target sequence (PAM is underlined) | Site of integration (in or before amino acid) | MIT score (in rat) | Conservation of target sequence in mouse | Source |
|---|---|---|---|---|---|---|
| Actb | β-actin | TGTGCCTTGATAGTTCGCCATGG | 1 bp before ATG | 84 | 1 mismatch | Willems et al. (2020) |
| Cacna1e | CaV2.3 (R-type) | CAGGATGGCTCGCTTCGGGGAGG | G5 | 88 | Yes | Willems et al. (2020) |
| Dlg4 | PSD95 | AATCAGAGTCTCTCTCGGGCTGG | R721 | 80 | Yes | Willems et al. (2020) |
| Gria1 | GluA1 | GGGAGCCACAGGATTGTAACTGG | STOP codon | 72 | Yes | Willems et al. (2020) |
| Homer1 | Homer1 | TATATGCCTCCGGTGCCCAGAGG | G2 | 49 | Yes | This study |
| Kcnma1 | BK | GAACGTACTTCTGTTTGTCGGGC | D1201 | 86 | Yes | This study |
| Kcnn2 | SK2 | TAGCTACTCTCAGATGAAGTTGG | T571 | 68 | 1 mismatch | This study |
| Mpp2 | MPP2 | TCAGAGTTCGTGGCAGCAACCGG | A4 | 74 | 1 mismatch | This study |
| Tubb3 | β3-tubulin | GCTGCGAGCAACTTCACTTGGG | STOP codon | 59 | Yes | Willems et al. (2020) |
| Grin1 | GluN1 | CTTGGGGTCGCAGGCGGCGCTGG | A20 | 85 | Yes | Willems et al. (2020) |
All knock-ins that were used in this study are listed with their gene and protein name, target sequence and target properties.