Statistical analysis for experiments 1–4 (SPSS GLM repeated-measures module)
| Figure number | Factor name | F value | P value | Partial Eta2 |
|---|---|---|---|---|
| Figure 1B, self-administration Repeated-measures ANOVA | With sex as a factor Food Sex (male, female), between-subjects Session (1–6), within-subjects Sex × Session interaction Fentanyl Sex (male, female), between-subjects Session (1–12), within-subjects Sex × Session interaction | F(1,12) = 1.0 F(5,60) = 1.1 F(5,60) = 0.1 F(1,12) = 0.7 F(11,132) = 3.7 F(11,132) = 1.4 | 0.35 0.37 0.99 0.43 <0.001* 0.20 | 0.07 0.08 0.01 0.05 0.24 0.10 |
| Figure 1C, discrete choice Repeated-measures ANOVA | With sex as a factor Preference score Sex (male, female), between-subjects Session (1–12), within-subjects Sex × Session interaction | F(1,12) = 4.8 F(11,132) = 15.4 F(11,132) = 1.0 | 0.05* <0.001* 0.45 | 0.28 0.56 0.08 |
| Figure 1D, relapse test Total responding Repeated-measures ANOVA | Without sex as a factor Lever (active, inactive), within-subjects | F(1,7) = 37.0 | <0.001* | 0.84 |
| Figure 1D, relapse test Time course Repeated-measures ANOVA | Without sex as a factor Session Time (15, 30, 45, 60), within-subjects Lever (active, inactive), within-subjects Session Time × Lever interaction | F(3,21) = 11.1 F(1,7) = 37.0 F(3,21) = 9.6 | <0.001* <0.001* <0.001* | 0.61 0.84 0.58 |
| Figure 1F, Fos neuron counting Repeated-measures ANOVA | OFC Cnr1: without sex as a factor Fos Test Condition (Test, No Test), between-subjects Cnr1 Test Condition (Test, No Test), between-subjects Fos+Cnr1 Test Condition (Test, No Test), between-subjects Pir Cnr1: without sex as a factor Fos Test Condition (Test, No Test), between-subjects Cnr1 Test Condition (Test, No Test), between-subjects Fos+Cnr1 Test Condition (Test, No Test), between-subjects OFC Cnr1 and Vgat: without sex as a factor Cnr1+Vgat Test Condition (Test, No Test), between-subjects Fos+Cnr1+Vgat Test Condition (Test, No Test), between-subjects OFC Drd1 and Drd2: without sex as a factor Fos Test Condition (Test, No Test), between-subjects Drd1 Test Condition (Test, No Test), between-subjects Drd2 Test Condition (Test, No Test), between-subjects Fos+Drd1 Test Condition (Test, No Test), between-subjects Fos+Drd2 Test Condition (Test, No Test), between-subjects Pir Drd1 and Drd2: without sex as a factor Fos Test Condition (Test, No Test), between-subjects Drd1 Test Condition (Test, No Test), between-subjects Drd2 Test Condition (Test, No Test), between-subjects Fos+Drd1 Test Condition (Test, No Test), between-subjects Fos+Drd2 Test Condition (Test, No Test), between-subjects | F(1,12) = 10.4 F(1,12) = 2.4 F(1,12) = 11.7 F(1,12) = 5.1 F(1,12) = 0.0 F(1,12) = 1.6 F(1,12) = 0.3 F(1,12) = 6.2 F(1,10) = 5.4 F(1,10) = 2.9 F(1,10) = 1.4 F(1,10) = 1.6 F(1,10) = 2.2 F(1,12) = 7.2 F(1,12) = 0.0 F(1,12) = 1.7 F(1,12) = 5.4 F(1,12) = 1.7 | 0.007* 0.15 0.005* 0.04* 0.89 0.23 0.57 0.03* 0.04* 0.12 0.27 0.24 0.17 0.02* 0.99 0.22 0.04* 0.22 | 0.47 0.17 0.49 0.30 0.00 0.12 0.03 0.34 0.35 0.22 0.12 0.14 0.18 0.37 0.00 0.12 0.31 0.13 |
| Figure 2B, self-administration Repeated-measures ANOVA | With sex as a factor Food Sex (male, female), between-subjects Session (1–6), within-subjects Sex × Session interaction Fentanyl Sex (male, female), between-subjects Session (1–12), within-subjects Sex × Session interaction | F(1,44) = 0.2 F(5,220) = 12.4 F(5,220) = 4.6 F(1,44) = 0.8 F(11,484) = 32.0 F(11,484) = 0.7 | 0.69 <0.001* <0.001* 0.38 <0.001* 0.74 | 0.00 0.22 0.10 0.02 0.42 0.02 |
| Figure 2C, discrete choice Repeated-measures ANOVA | With sex as a factor Preference score Sex (male, female), between-subjects Session (1–12), within-subjects Sex × Session interaction | F(1,44) = 12.3 F(11,484) = 15.2 F(11,484) = 1.7 | 0.001* <0.001* 0.07 | 0.22 0.26 0.04 |
| Figure 2D, relapse test Total responding Mixed ANOVA | With sex as a factor Sex (male, female), between-subjects AM251 Dose (0, 0.3, 1 µg), between-subjects Lever (active, inactive) within-subjects AM251 Dose × Lever interaction Sex × AM251 Dose interaction Sex × Lever interaction Sex × AM251 Dose × Lever interaction | F(1,40) = 0.0 F(2,40) = 1.0 F(1,40) = 152.7 F(2,40) = 0.9 F(2,40) = 0.3 F(1,40) = 0.0 F(2,40) = 1.2 | 0.94 0.39 <0.001* 0.43 0.74 0.97 0.31 | 0.0 0.05 0.79 0.04 0.02 0.0 0.06 |
| Figure 2D, relapse test Time course Mixed-ANOVA | Without sex as a factor AM251 Dose (0, 0.3, 1 µg), between-subjects Session Hour (1–3) within-subjects Lever (active, inactive), within-subjects AM251 Dose × Session Hour interaction AM251 Dose × Lever interaction Session Hour × Lever interaction AM251 Dose × Session Hour × Lever interaction | F(2,43) = 1.1 F(2,86) = 144.2 F(1,43) = 160.4 F(4,86) = 1.8 F(2,43) = 1.0 F(2,86) = 131.5 F(4,86) = 1.4 | 0.34 <0.001* <0.001* 0.14 0.39 <0.001* 0.24 | 0.05 0.77 0.79 0.08 0.04 0.75 0.06 |
| Figure 2E, reacquisition Mixed-ANOVA | With sex as a factor Sex (male, female), between-subjects AM251 Dose (0, 0.3, 1 µg), between-subjects Session Hour (1–6) within-subjects AM251 Dose × Session Hour interaction Sex × AM251 Dose interaction Sex × Session Hour interaction Sex × AM251 Dose × Session Hour interaction | F(1,40) = 1.9 F(2,40) = 1.2 F(5,200) = 8.7 F(10,200) = 1.3 F(2,40) = 2.9 F(5,200) = 1.9 F(10,200) = 1.2 | 0.18 0.30 <0.001* 0.26 0.07 0.10 0.32 | 0.04 0.06 0.18 0.06 0.13 0.04 0.06 |
| Figure 3B, self-administration Repeated-measures ANOVA | Without sex as a factor Food Session (1–6), within-subjects Fentanyl Session (1–12), within-subjects | F(5,50) = 1.5 F(11,110) = 5.3 | 0.22 <0.001* | 0.13 0.35 |
| Figure 3C, discrete choice Repeated-measures ANOVA | Without sex as a factor Preference score Session (1–12), within-subjects | F(11,110) = 2.7 | 0.004* | 0.22 |
| Figure 3D, relapse test Total responding Repeated-measures ANOVA | Without sex as a factor (without statistical outlier) WIN55,212-2 Injection (vehicle, WIN55,212-2), within-subjects WIN55,212-2 Dose (0.3, 1 µg), between-subjects Lever (active, inactive) within-subjects WIN55,212-2 Injection × Dose interaction WIN55,212-2 Injection × Lever interaction WIN55,212-2 Dose × Lever interaction WIN55,212-2 Injection × Dose × Lever interaction | F(1,8) = 0.4 F(1,8) = 0.0 F(1,8) = 38.4 F(1,8) = 0.0 F(1,8) = 0.6 F(1,8) = 0.0 F(1,8) = 0.0 | 0.57 0.87 <0.001* 0.86 0.46 0.94 0.85 | 0.04 0.00 0.83 0.00 0.07 0.00 0.00 |
| Figure 3D, relapse test Total responding Repeated-measures ANOVA | Without sex as a factor (with statistical outlier) WIN55,212-2 Injection (vehicle, WIN55,212-2), within-subjects WIN55,212-2 Dose (0.3, 1 µg), between-subjects Lever (active, inactive) within-subjects WIN55,212-2 Injection × Dose interaction WIN55,212-2 Injection × Lever interaction WIN55,212-2 Dose × Lever interaction WIN55,212-2 Injection × Dose × Lever interaction | F(1,9) = 0.1 F(1,9) = 0.6 F(1,9) = 26.5 F(1,9) = 0.7 F(1,9) = 0.1 F(1,9) = 0.5 F(1,9) = 0.8 | 0.75 0.45 <0.001* 0.42 0.73 0.48 0.40 | 0.01 0.07 0.75 0.07 0.01 0.06 0.08 |
| Figure 3E, reacquisition Repeated-measures ANOVA | Without sex as a factor WIN55,212-2 Injection (vehicle, WIN55,212-2), within-subjects WIN55,212-2 Dose (0.3, 1 µg), between-subjects WIN55,212-2 Injection × Dose interaction | F(1,9) = 0.6 F(1,9) = 4.5 F(1,9) = 0.3 | 0.44 0.06 0.61 | 0.07 0.33 0.03 |
| Figure 3G, re-training Repeated-measures ANOVA | Without sex as a factor Fentanyl Session (1–4), within-subjects | F(3,30) = 0.2 | 0.92 | 0.02 |
| Figure 3H, discrete choice Repeated-measures ANOVA | Without sex as a factor Preference score Session (1–4), within-subjects | F(3,30) = 1.5 | 0.25 | 0.13 |
| Figure 4B, self-administration Repeated-measures ANOVA | With sex as a factor Food Sex (male, female), between-subjects Session (1–6), within-subjects Sex × Session interaction Fentanyl Sex (male, female), between-subjects Session (1–12), within-subjects Sex × Session interaction | F(1,18) = 1.3 F(5,90) = 3.9 F(5,90) = 5.8 F(1,18) = 0.0 F(11,198) = 2.9 F(11,198) = 0.5 | 0.27 0.003* <0.001* 0.97 0.001* 0.89 | 0.07 0.18 0.24 0.00 0.14 0.03 |
| Figure 4C, discrete choice Repeated-measures ANOVA | With sex as a factor Preference score Sex (male, female), between-subjects Session (1–12), within-subjects Sex × Session interaction | F(1,18) = 0.2 F(11,198) = 5.9 F(11,198) = 1.5 | 0.66 <0.001* 0.12 | 0.01 0.25 0.08 |
| Figure 4D, relapse test Total responding Repeated-measures ANOVA | Without sex as a factor (without statistical outlier) SCH39166 Injection (vehicle, SCH39166), within-subjects SCH39166 Dose (1, 3 µg), between-subjects Lever (active, inactive) within-subjects SCH39166 Injection × Dose interaction SCH39166 Injection × Lever interaction SCH39166 Dose × Lever interaction SCH39166 Injection × Dose × Lever interaction | F(1,17) = 0.0 F(1,17) = 0.9 F(1,17) = 130.4 F(1,17) = 0.2 F(1,17) = 0.1 F(1,17) = 4.6 F(1,17) = 0.0 | 0.86 0.35 <0.001* 0.65 0.82 0.05* 0.93 | 0.00 0.05 0.89 0.01 0.00 0.21 0.00 |
| Figure 4D, relapse test Total responding Repeated-measures ANOVA | Without sex as a factor (with statistical outlier) SCH39166 Injection (vehicle, SCH39166), within-subjects SCH39166 Dose (1, 3 µg), between-subjects Lever (active, inactive) within-subjects SCH39166 Injection × Dose interaction SCH39166 Injection × Lever interaction SCH39166 Dose × Lever interaction SCH39166 Injection × Dose × Lever interaction | F(1,18) = 0.6 F(1,18) = 0.0 F(1,18) = 44.2 F(1,18) = 0.9 F(1,18) = 0.3 F(1,18) = 0.2 F(1,18) = 0.5 | 0.46 0.99 <0.001* 0.36 0.61 0.68 0.48 | 0.03 0.00 0.71 0.05 0.02 0.01 0.03 |
| Figure 4E, reacquisition Repeated-measures ANOVA | Without sex as a factor SCH39166 Injection (vehicle, SCH39166), within-subjects SCH39166 Dose (1, 3 µg), between-subjects SCH39166 Injection × Dose interaction | F(1,18) = 0.2 F(1,18) = 1.8 F(1,18) = 0.1 | 0.63 0.20 0.77 | 0.01 0.09 0.01 |
| Figure 4G, re-training repeated-measures ANOVA | With sex as a factor Fentanyl Sex (male, female), between-subjects Session (1–2), within-subjects Sex × Session interaction | F(1,18) = 2.2 F(1,18) = 1.9 F(1,18) = 0.4 | 0.15 0.19 0.55 | 0.11 0.09 0.02 |
| Figure 4H, discrete choice Repeated-measures ANOVA | With sex as a factor Preference score Sex (male, female), between-subjects Session (1–4), within-subjects Sex × Session interaction | F(1,18) = 3.2 F(3,54) = 0.1 F(3,54) = 0.1 | 0.09 0.93 0.94 | 0.15 0.01 0.01 |
Partial Eta2 = proportion of explained variance.