Table 1

Two-way ANOVA results of Fos quantification in analyzed brain regions following hyperalgesia during spontaneous withdrawal

Brain regionTreatment effectSex effectSex × treatment interaction
NAc shellF(1,19) = 0.8349, p = 0.3723F(1,19) = 10.41, p = 0.0044; F > MF(1,19) = 0.05892, p = 0.8108
BNSTF(1,19) = 1.926, p = 0.1813F(1,19) = 4.275, p = 0.0526F(1,19) = 0.4395, p = 0.5153
PVNF(1,19) = 30.16, p < 0.0001F(1,19) = 8.964, p = 0.0075; F > MF(1,19) = 0.2445, p = 0.1344
PVTF(1,19) = 0.1164, p = 0.7367F(1,19) = 2.544, p = 0.1272F(1,19) = 0.2627, p = 0.6142
LHF(1,19) = 16.93, p < 0.001F(1,19) = 5.546, p = 0.0294; F > MF(1,19) = 0.1327, p = 0.7197
CeAF(1,19) = 26.37, p < 0.0001F(1,19) = 1.876, p = 0.1867F(1,19) = 0.09373, p = 0.7628
LHbF(1,19) = 40.80, p < 0.0001F(1,19) = 8.195, p = 0.0100; F > MF(1,19) = 0.9071, p = 0.3528
PAGF(1,19) = 1.136, p = 0.2998F(1,19) = 8.589, p = 0.0086; F > MF(1,19) = 0.005, p = 0.9441
SUMF(1,19) = 4.195, p = 0.0546F(1,19) = 14.44, p = 0.0012; F > MF(1,19) = 0.007174, p = 0.9334
VTAF(1,19) = 27.39, p < 0.0001F(1,19) = 5.773, p = 0.0267; F > MF(1,19) = 0.1231, p = 0.2810
DRF(1,19) = 11.70, p < 0.01F(1,19) = 1.803, p = 0.1952F(1,19) = 0.08641, p = 0.3642
PBNF(1,19) = 26.15, p < 0.0001F(1,19) = 0.2822, p = 0.6014F(1,19) = 0.1759, p = 0.6796
LCF(1,19) = 17.91, p < 0.001F(1,19) = 0.5460, p = 0.4695F(1,19) = 0.5909, p = 0.4520
  • Males (M), Females (F).