Table 1

Materials and chemicals used for dissection and electrophysiological recordings

ItemManufacturerNotes
Fluorescent stereomicroscope, for larval dissectionLeica MZ10-F, or equivalentEquipped with a plan apo 1× objective, fluorescent lamp, and appropriate filters for fluorescence
Upright microscopeZeiss Axioscope, or equivalentEquipped with 10×, 20×, and 40× objectives; microscope should be also equipped with DIC/IR optics
Micromanipulator/sSutter Instruments MP-285, or equivalent
Amplifier with A/D converter and recording software connected to computerMolecular Devices Multiclamp 200 series amplifier, or equivalentComputer equipped with P-Clamp acquisition and analysis software or equivalent
StimulatorDigitimer DS3, or equivalentConstant current isolated stimulator
Borosilicate capillary glass for recordingsWorld Precision InstrumentsThinwall glass OD/ID 1.5/1.2 mm (catalog #TW150F-3)
Theta glassWorld Precision InstrumentsFor stimulating electrodes OD/ID 1.5/1.02 mm (catalog #TST150-6)
Transfer pipettesFisher ScientificPipettes to transfer larval zebrafish (catalog #13-678-20A)
Electrode pipette pullerSutter Instruments P-97, or equivalent
Fluorodish cell culture dish 35 mm, 10 mm wellWorld Precision InstrumentsCell culture dish with glass bottom covered with Sylgard (catalog #FD3510-100)
Mini-Petri dishesSigma60-mm diameter (catalog #5481-500EA)
MS-222SigmaMS-222 (0.03%) solution buffered with sodium bicarbonate, pH 7.4 (adjusted with NaOH)
ForcepsFine Science ToolsDumont #4 Forceps (catalog #11242-40)
Tungsten wire to make custom-made pinsAM SystemsTungsten wire, size 0.002 inches bare diameter (catalog #795500)
Tungsten wire to make the dissection pinAM SystemsTungsten wire, size 0.005 inches bare diameter (catalog #797600)
MASigmaCatalog #6385-02-0
CNQXTocrisCatalog #0190
DAP5TocrisCatalog #0106
Pneumatic Transducer TesterFluke BiomedicalDPM1B
d-tubocurarineSigma10–15 μm d-tubocurarine in external solution (in mm): 134 NaCl, 2.9 KCl, 2.1 CaCl2, 1.2 MgCl2, 10 HEPES, and 10 glucose, pH adjusted to 7.8 with NaOH