The effects of TBOA on AII responses and RB membrane potentials
Data | Data structure | Type of test | Power | Mean ± SEM | Number of cells | |
---|---|---|---|---|---|---|
Effect of TBOA on AII EPSC amplitude (unit: pA) | ||||||
Control | Normal distribution | −237.40 ± 16.21 | 7 | |||
50 μm TBOA | Non-normal distribution | −547.20 ± 61.50 | 7 | |||
a | Control vs TBOA | Wilcoxon signed-rank test | p = 0.0156 | 7 | ||
Relative effects of TBOA on AII EPSC amplitude and integral | ||||||
Control | Non-normal distribution | 1.00 ± 0.00 | 7 | |||
50 μm TBOA (amplitude) | Normal distribution | 2.31 ± 0.20 | 7 | |||
50 μm TBOA (integral) | Non-normal distribution | 11.22 ± 1.70 | 7 | |||
b | Control vs TBOA (amplitude) | Wilcoxon signed-rank test | p = 0.0156 | 7 | ||
c | Control vs TBOA (integral) | Wilcoxon signed-rank test | p = 0.0156 | 7 | ||
d | TBOA (amplitude) vs TBOA (integral) | Wilcoxon signed-rank test | p = 0.0156 | 7 | ||
Relative effect of DHK on AII EPSC amplitude | ||||||
200 μm DHK | Normal distribution | 1.18 ± 0.04 | 7 | |||
e | DHK vs TBOA (amplitude) | Unpaired Student’s t test | p = 0.0001 | |||
Effect of TBOA on AII EPSC time to peak (unit: ms) | ||||||
Control | Normal distribution | 14.16 ± 0.62 | 7 | |||
50 μm TBOA | Normal distribution | 26.21 ± 2.12 | 7 | |||
f | Control vs TBOA | Paired Student’s t test | p = 0.0004 | 7 | ||
Effect of TBOA on AII EPSC rise time (unit: ms) | ||||||
Control | Normal distribution | 3.34 ± 0.75 | 7 | |||
50 μm TBOA | Normal distribution | 10.23 ± 1.99 | 7 | |||
g | Control vs TBOA | Paired Student’s t test | p = 0.0032 | 7 | ||
Effect of TBOA on AII EPSC tau (unit: ms) | ||||||
Control | Non-normal distribution | 64.54 ± 20.61 | 7 | |||
50 μm TBOA | Normal distribution | 325 ± 68.02 | 7 | |||
h | Control vs TBOA | Paired Student’s t test | p = 0.0050 | 7 | ||
Effect of TBOA on AII mEPSC frequency (unit: Hz) | ||||||
Control | Normal distribution | 6.19 ± 1.14 | 7 | |||
50 μm TBOA | Normal distribution | 6.19 ± 1.19 | 7 | |||
i | Control vs TBOA | Paired Student’s t test | p = 0.9854 | 7 | ||
Effect of TBOA on AII mEPSC amplitude (unit: pA) | ||||||
Control | Normal distribution | −19.98 ± 1.55 | 7 | |||
50 μm TBOA | Normal distribution | −20.13 ± 1.57 | 7 | |||
j | Control vs TBOA | Paired Student’s t test | p = 0.8856 | 7 | ||
Effect of TBOA on AII mEPSC rise time (unit: ms) | ||||||
Control | Non-normal distribution | 0.32 ± 0.01 | 7 | |||
50 μm TBOA | Normal distribution | 0.32 ± 0.01 | 7 | |||
k | Control vs TBOA | Wilcoxon signed-rank test | p > 0.9999 | 7 | ||
Effect of TBOA on AII mEPSC tau (unit: ms) | ||||||
Control | Normal distribution | 0.75 ± 0.04 | 7 | |||
50 μm TBOA | Normal distribution | 0.81 ± 0.06 | 7 | |||
l | Control vs TBOA | Paired Student’s t test | p = 0.0392 | 7 | ||
Effect of TBOA on RB light-evoked voltage change (unit: mV) | ||||||
Control | Normal distribution | 8.69 ± 0.39 | 5 | |||
50 μm TBOA | Normal distribution | 9.64 ± 0.51 | 5 | |||
m | Control vs TBOA | Paired Student’s t test | p = 0.0192 | 5 | ||
Effect of TBOA on RB resting membrane potential (unit: mV) | ||||||
Control | Normal distribution | −52.92 ± 2.01 | 5 | |||
50 μm TBOA | Normal distribution | −52.91 ± 2.95 | 5 | |||
n | Control vs TBOA | Paired Student’s t test | p = 0.9931 | 5 | ||
Relative effects of DHK and TBOA on RB light-evoked voltage change | ||||||
200 μm DHK | Normal distribution | 1.16 ± 0.03 | 6 | |||
50 μm TBOA | Normal distribution | 1.11 ± 0.03 | 5 | |||
o | DHK vs TBOA | Unpaired Student’s t test | p = 0.3226 |
TBOA, a non-selective EAAT blocker; DHK, a selective EAAT2 blocker; AII, AII amacrine cell; RB, rod bipolar cell.