Table 5

The effects of TBOA on AII responses and RB membrane potentials

DataData structureType of testPowerMean ± SEMNumber
of cells
Effect of TBOA on AII EPSC amplitude (unit: pA)
ControlNormal distribution−237.40 ± 16.217
50 μm TBOANon-normal distribution−547.20 ± 61.507
aControl vs TBOAWilcoxon signed-rank testp = 0.01567
Relative effects of TBOA on AII EPSC amplitude and integral
ControlNon-normal distribution1.00 ± 0.007
50 μm TBOA (amplitude)Normal distribution2.31 ± 0.207
50 μm TBOA (integral)Non-normal distribution11.22 ± 1.707
bControl vs TBOA (amplitude)Wilcoxon signed-rank testp = 0.01567
cControl vs TBOA (integral)Wilcoxon signed-rank testp = 0.01567
dTBOA (amplitude) vs TBOA (integral)Wilcoxon signed-rank testp = 0.01567
Relative effect of DHK on AII EPSC amplitude
200 μm DHKNormal distribution1.18 ± 0.047
eDHK vs TBOA (amplitude)Unpaired Student’s t testp = 0.0001
Effect of TBOA on AII EPSC time to peak (unit: ms)
ControlNormal distribution14.16 ± 0.627
50 μm TBOANormal distribution26.21 ± 2.127
fControl vs TBOAPaired Student’s t testp = 0.00047
Effect of TBOA on AII EPSC rise time (unit: ms)
ControlNormal distribution3.34 ± 0.757
50 μm TBOANormal distribution10.23 ± 1.997
gControl vs TBOAPaired Student’s t testp = 0.00327
Effect of TBOA on AII EPSC tau (unit: ms)
ControlNon-normal distribution64.54 ± 20.617
50 μm TBOANormal distribution325 ± 68.027
hControl vs TBOAPaired Student’s t testp = 0.00507
Effect of TBOA on AII mEPSC frequency (unit: Hz)
ControlNormal distribution6.19 ± 1.147
50 μm TBOANormal distribution6.19 ± 1.197
iControl vs TBOAPaired Student’s t testp = 0.98547
Effect of TBOA on AII mEPSC amplitude (unit: pA)
ControlNormal distribution−19.98 ± 1.557
50 μm TBOANormal distribution−20.13 ± 1.577
jControl vs TBOAPaired Student’s t testp = 0.88567
Effect of TBOA on AII mEPSC rise time (unit: ms)
ControlNon-normal distribution0.32 ± 0.017
50 μm TBOANormal distribution0.32 ± 0.017
kControl vs TBOAWilcoxon signed-rank testp > 0.99997
Effect of TBOA on AII mEPSC tau (unit: ms)
ControlNormal distribution0.75 ± 0.047
50 μm TBOANormal distribution0.81 ± 0.067
lControl vs TBOAPaired Student’s t testp = 0.03927
Effect of TBOA on RB light-evoked voltage change (unit: mV)
ControlNormal distribution8.69 ± 0.395
50 μm TBOANormal distribution9.64 ± 0.515
mControl vs TBOAPaired Student’s t testp = 0.01925
Effect of TBOA on RB resting membrane potential (unit: mV)
ControlNormal distribution−52.92 ± 2.015
50 μm TBOANormal distribution−52.91 ± 2.955
nControl vs TBOAPaired Student’s t testp = 0.99315
Relative effects of DHK and TBOA on RB light-evoked voltage change
200 μm DHKNormal distribution1.16 ± 0.036
50 μm TBOANormal distribution1.11 ± 0.035
oDHK vs TBOAUnpaired Student’s t testp = 0.3226
  • TBOA, a non-selective EAAT blocker; DHK, a selective EAAT2 blocker; AII, AII amacrine cell; RB, rod bipolar cell.