Data | Data structure | Type of test | Power | Mean ± SEM | Number of cells | |
---|---|---|---|---|---|---|
Effects of DHK, UCPH101 and TBOA on the average amplitude of AII EPSPs at various stimulus frequencies | ||||||
2-Hz stimulus frequency | ||||||
Control | Normal distribution | 15.75 ± 1.77 | 7 | |||
200 μm DHK + 50 μm UCPH101 | Normal distribution | 14.62 ± 1.77 | 7 | |||
200 μm DHK + 50 μm UCPH101 + 50 μm TBOA | Normal distribution | 5.44 ± 0.97 | 7 | |||
a | Control vs DHK + UCPH | Paired t test | p = 0.1704 | 7 | ||
b | DHK + UCPH vs DHK + UCPH + TBOA | Paired t test | p = 0.0013 | 7 | ||
5-Hz stimulus frequency | ||||||
Control | Normal distribution | 14.58 ± 1.16 | 7 | |||
200 μm DHK + 50 μm UCPH101 | Normal distribution | 14.36 ± 1.48 | 7 | |||
200 μm DHK + 50 μm UCPH101 + 50 μm TBOA | Normal distribution | 5.35 ± 0.62 | 7 | |||
c | Control vs DHK + UCPH | Paired t test | p = 0.7810 | 7 | ||
d | DHK + UCPH vs DHK + UCPH + TBOA | Paired t test | p = 0.0007 | 7 | ||
10-Hz stimulus frequency | ||||||
Control | Normal distribution | 11.90 ± 1.66 | 7 | |||
200 μm DHK + 50 μm UCPH101 | Normal distribution | 10.74 ± 1.79 | 7 | |||
200 μm DHK + 50 μm UCPH101 + 50 μm TBOA | Normal distribution | 2.70 ± 0.52 | 7 | |||
e | Control vs DHK + UCPH | Paired t test | p = 0.0746 | 7 | ||
f | DHK + UCPH vs DHK + UCPH + TBOA | Paired t test | p = 0.0054 | 7 | ||
20-Hz stimulus frequency | ||||||
Control | Normal distribution | 13.41 ± 1.74 | 7 | |||
200 μm DHK + 50 μm UCPH101 | Normal distribution | 12.45 ± 2.42 | 7 | |||
200 μm DHK + 50 μm UCPH101 + 50 μm TBOA | Normal distribution | 4.12 ± 0.29 | 7 | |||
g | Control vs DHK + UCPH | Paired t test | p = 0.4964 | 7 | ||
h | DHK + UCPH vs DHK + UCPH + TBOA | Paired t test | p = 0.0116 | 7 | ||
50-Hz stimulus frequency | ||||||
Control | Normal distribution | 9.99 ± 1.42 | 7 | |||
200 μm DHK + 50 μm UCPH101 | Normal distribution | 8.67 ± 1.65 | 7 | |||
200 μm DHK + 50 μm UCPH101 + 50 μm TBOA | Non-normal distribution | 3.58 ± 0.24 | 7 | |||
i | Control vs DHK + UCPH | Paired t test | p = 0.0763 | 7 | ||
j | DHK + UCPH vs DHK + UCPH + TBOA | Wilcoxon signed-rank test | p = 0.0156 | 7 | ||
Effects of DHK alone on the average amplitude of AII EPSPs at various stimulus frequencies | ||||||
2-Hz stimulus frequency | ||||||
Control | Normal distribution | 14.33 ± 3.61 | 5 | |||
200 μm DHK | Normal distribution | 13.46 ± 2.41 | 5 | |||
k | Control vs DHK | Paired t test | p = 0.6330 | 5 | ||
5-Hz stimulus frequency | ||||||
Control | Normal distribution | 13.25 ± 1.97 | 5 | |||
200 μm DHK | Normal distribution | 12.57 ± 1.71 | 5 | |||
l | Control vs DHK | Paired t test | p = 0.5547 | 5 | ||
10-Hz stimulus frequency | ||||||
Control | Normal distribution | 11.26 ± 1.87 | 5 | |||
200 μm DHK | Normal distribution | 10.65 ± 1.55 | 5 | |||
m | Control vs DHK | Paired t test | p = 0.4382 | 5 | ||
20-Hz stimulus frequency | ||||||
Control | Normal distribution | 11.26 ± 0.76 | 5 | |||
200 μm DHK | Normal distribution | 11.51 ± 1.08 | 5 | |||
n | Control vs DHK | Paired t test | p = 0.8054 | 5 | ||
50-Hz stimulus frequency | ||||||
Control | Normal distribution | 8.74 ± 1.07 | 5 | |||
200 μm DHK | Normal distribution | 7.88 ± 1.05 | 5 | |||
o | Control vs DHK | Paired t test | p = 0.0780 | 5 |
TBOA, a non-selective EAAT blocker; UCPH101, a selective EAAT1/GLAST blocker; DHK, a selective EAAT2 blocker; AII, AII amacrine cell.