Table 2

List of the reagents and antibodies used in our modified version of the iDISCO+ protocol for staining Fos in intact mouse brains

ReagentDetailsSupplier
Phospho-c-Fos (Ser32) (D82C12) XP rabbit mAb5348S
(lot #1)
Cell Signaling
Anti-GFP/YFP (GFP antibody)GFP-1020
(lot #GFP697986)
Aves Laboratory
Alexa Fluor 647 AffiniPure F(ab')2, fragment donkey
anti-rabbit IgG (H+L)
711-606-152
(lot #128806)
Jackson ImmunoResearch
Alexa Fluor 488 AffiniPure F(ab')2, fragment donkey
anti-chicken IgY (IgG; H+L)
703-546-155
(lot #127495)
Jackson ImmunoResearch
10× PBS119-069101Quality Biological
PFA (prilled)19202Electron Microscopy Science
Sodium phosphate (monobasic monohydrate)BDH9298VWR Life Science
Tween 20P9416Sigma-Aldrich
Triton X-100X100Sigma-Aldrich
DCM270997Sigma-Aldrich
MeOHA412SKFisher
DMSOD128-4Fisher Scientific
GlycineG7126Sigma-Aldrich
HeparinH3393Sigma-Aldrich
Sodium azide58032Sigma-Aldrich
H2O2 30%216763Sigma
NDS017-000-121Jackson ImmunoResearch
Benzyl ether 98%10801Sigma-Aldrich
Falcon 15-ml centrifuge tubes (polystyrene)05-527-90Fisher Scientific
Wide mouth amber vial (26 × 26 mm)CT242626-A-TEF-144Discount Vials
Corning 1.2-ml cryogenic vials with external threadCLS430658-500EASigma-Aldrich
  • Supplier and catalog number are listed; lot numbers are provided when possible.