Table 2

Other reagents used

ItemVendorProduct
number
RRID or link
NestinCreERT2 miceThe Jackson
Laboratory
#016261IMSR_JAX:016261
R26R-LoxP-STOP-
LoxP-EYFP
The Jackson
Laboratory
#007909IMSR_JAX:007909
BrdUSigma#B5002https://www.sigmaaldrich.com/US/en/product/sigma/b5002?gclid=Cj0KCQiAjJOQBhCkARIsAEKMtO2Z8yDVbyW9L9oduQd8T7l-a8P6oFzW-2kFd-oo4Nm87mNJH-mzwZQaAgXJEALw_wcB
EdUClick Chemistry
Tools
#1149-500https://clickchemistrytools.com/product/5-ethynyl-2%E2%80%B2-deoxyuridine-edu/
TAM chowEnvigoTD.130858https://www.envigo.com/tamoxifen-custom-diets
ParaformaldehydeFisher#AC169650010https://www.fishersci.com/shop/products/paraformaldehyde-90-pure-thermo-scientific/AC169650010
SucroseFisherS5-3https://www.fishersci.com/shop/products/sucrose-crystalline-certified-acs-fisher-chemical-3/S53#?keyword=S5-3
PBSFisher#BP399-20https://www.fishersci.com/shop/products/pbs-phosphate-buffered-saline-10x-solution-fisher-bioreagents/BP39920?searchHijack=true&searchTerm=BP399-20&searchType=RAPID&matchedCatNo=BP399-20
Normal donkey
serum
Jackson
ImmunoResearch
#017000121AB_2337258
Triton X-100Fisher#AAA16046AEhttps://www.fishersci.com/shop/products/triton-x-100-thermo-scientific/AAA16046AE?searchHijack=true&searchTerm=triton-x-100-thermo-scientific&searchType=Rapid&matchedCatNo=AAA16046AE
Hoechst 33342Fisher#H3570https://www.fishersci.com/shop/products/molecular-probes-hoechst-33342-trihydrochloride-trihydrate/H3570#?keyword=H3570
SuperFrost
Plus slides
Fisher#12-550-15https://www.fishersci.com/shop/products/fisherbrand-superfrost-plus-microscope-slides-2/1255015#?keyword=12-550-15
Prolong Gold Antifade
mounting medium
Fisher#P36934https://www.fishersci.com/shop/products/molecular-probes-prolong-gold-antifade-mountant-5/P36934?searchHijack=true&searchTerm=P36934&searchType=RAPID&matchedCatNo=P36934
12N HClFisherA144500https://www.fishersci.com/shop/products/hydrochloric-acid-certified-acs-plus-fisher-chemical-10/A144500#?keyword=A144500
Click&Go EdU
488 imaging kit
Click Chemistry
Tools
#1324https://clickchemistrytools.com/product/click-go-edu-488-imaging-kit/
  • List of other major reagents.