Table 1

Statistical tests used to analyze data

Circuit
location		ComparisonData
structure		TestSample size
(# KC fields or
Western lysates)		MeanStatisticsp value
α’10 dpe; 7 dpeNot normalMann–Whitney test38; 340.1; 32.5U = 0p < 0.0001
β’10 dpe; 7 dpeNot normalMann–Whitney test37; 380.1; 66.8U = 0p < 0.0001
α’1Female 7 dpe; male 7 dpenormalUnpaired two-tailed t test18; 1650.1; 26.9t = 6.51 df = 26.96p < 0.0001
β’1Female 7 dpe; male 7 dpeNot normalMann–Whitney test18; 1683.2; 64.5U = 31p < 0.0001
N/AFemale 0 dpe; male 0 dpeNormalBrown–Forsythe, Welch ANOVA test9; 91.0; 1.104t = 2.028 DF = 15.75p = 0.2846
N/AFemale 0 dpe; female 7 dpeNormalBrown–Forsythe, Welch ANOVA test9; 91.0; 1.088t = 0.8219 DF = 10.31p = 0.9482
N/AFemale 0 dpe; male 7 dpeNormalBrown–Forsythe, Welch ANOVA test9; 91.0; 0.9298t = 0.7746 DF = 11.32p = 0.9608
N/AMale 0 dpe; female 7 dpeNormalBrown–Forsythe, Welch ANOVA test9; 91.104; 1.088t = 0.1493 DF = 9.809p > 0.9999
N/AMale 0 dpe; male 7 dpeNormalBrown–Forsythe, Welch ANOVA test9; 91.104; 0.9298t = 1.956 DF = 10.63p = 0.3383
N/AFemale 7 dpe; male 7 dpeNormalBrown–Forsythe, Welch ANOVA test9; 91.088; 0.9298t = 1.223 DF = 15.43p = 0.7724
α’1Control 7dpe; dfmr1 7dpeNormalUnpaired two-tailed t test34; 2143.5; 26.1t = 3.77 df = 41.34p = 0.0005
β’1Control 7dpe; dfmr1 7dpeNormalUnpaired two-tailed t test34; 2175.5; 43.9t = 3.88 df = 34.73p = 0.0004
α’1Control 7dpe; rg 7 dpeNot normalMann–Whitney test24; 1827.4; 15.2U = 70.50p = 0.0001
β’1Control 7dpe; rg 7 dpeNormalUnpaired two-tailed t test24; 1858.9; 20.8t = 8.40 df = 31.59p < 0.0001
α’1Control 0 dpe; control 7 dpeNot normalMann–Whitney test14; 120.1; 35.7U = 0p < 0.0001
α’1dFMRP OE 0 dpe; dFMRP OE 7 dpeNot normalMann–Whitney test16; 185.2; 31.6U = 11.50p < 0.0001
α’1hFMRP OE 0 dpe; hFMRP OE 7 dpeNot normalMann–Whitney test18; 110.2; 39.8U = 0p < 0.0001
α’1ΔRGG-hFMRP OE 0 dpe; ΔRGG-hFMRP OE 7 dpeNot normalMann–Whitney test8; 180.1; 5.3U = 9.50p = 0.0001
α’1Rg OE 0 dpe; Rg OE 7 dpeNormalUnpaired two-tailed t test14; 160.3; 31.6t = 8.94 df = 15.04p < 0.0001
α’1Meng-Po OE 0 dpe; Meng-Po OE 7dpeNormalUnpaired two-tailed t test16; 1667.1; 196.4t = 7.88 df = 16.78p < 0.0001
α’1Control 0 dpe; dFMRP OE 0 dpeNot normalKruskal–Wallis test14; 160.1; 5.2Mean rank diff = −22.9p = 0.0272
α’1Control 0 dpe; hFMRP OE 0 dpeNot normalKruskal–Wallis test14; 180.1; 0.2Mean rank diff = −0.7143p > 0.9999
α’1Control 0 dpe; ΔRGG-hFMRP OE 0 dpeNot normalKruskal–Wallis test14; 80.1; 0.1Mean rank diff = 0.5357p > 0.9999
α’1Control 0 dpe; Rg OE 0 dpeNot normalKruskal–Wallis test14; 140.1; 0.3Mean rank diff = −4.286p > 0.9999
α’1Control 0 dpe; Meng-Po OE 0 dpeNot normalKruskal–Wallis test14; 160.1; 67.1Mean rank diff = −49.21p < 0.0001
α’1Control 7 dpe; dFMRP OE 7 dpeNormalBrown–Forsythe, Welch ANOVA test12; 1835.7; 31.6t = 0.74 DF = 23.56p = 0.9505
α’1Control 7 dpe; hFMRP OE 7 dpeNormalBrown–Forsythe, Welch ANOVA test12; 1135.7; 39.8t = 0.62 DF = 20.17p = 0.9751
α’1Control 7 dpe; ΔRGG-hFMRP OE 7 dpeNormalBrown–Forsythe, Welch ANOVA test12; 1835.7; 5.3t = 6.81 DF = 12.48p < 0.0001
α’1Control 7 dpe; Rg OE 7 dpeNormalBrown–Forsythe, Welch ANOVA test12; 1635.7; 31.6t = 0.74 DF = 22.91p = 0.9508
α’1Control 7 dpe; Meng-Po OE 7 dpeNormalBrown–Forsythe, Welch ANOVA test12; 1635.7;196.4t = 9.74 DF = 17.16p < 0.0001
β’1Control 0 dpe; control 7 dpeNot normalMann–Whitney test14; 120.1; 53.6U = 0p < 0.0001
β’1dFMRP OE 0 dpe; dFMRP OE 7 dpeNot normalMann–Whitney test16; 183.4; 19.6U = 48.50p = 0.0005
β’1hFMRP OE 0 dpe; hFMRP OE 7 dpeNot normalMann–Whitney test18; 111.0; 60.5U = 1p < 0.0001
β’1ΔRGG-hFMRP OE 0 dpe; ΔRGG-hFMRP OE 7 dpeNot normalMann–Whitney test8; 180.1; 7.1U = 0p < 0.0001
β’1Rg OE 0 dpe; Rg OE 7 dpeNormalUnpaired two-tailed t test14; 160.4; 25.7t = 9.12 df = 15.12p < 0.0001
β’1Meng-Po OE 0 dpe; Meng-Po OE 7dpeNormalUnpaired two-tailed t test16; 22138.9; 251.9t = 7.49 df = 26.44p < 0.0001
β’1Control 0 dpe; dFMRP OE 0 dpeNot normalKruskal–Wallis test14; 160.1; 3.4Mean rank diff = −18.48p = 0.1195
β’1Control 0 dpe; hFMRP OE 0 dpeNot normalKruskal–Wallis test14; 180.1; 1.0Mean rank diff = −6.127p > 0.9999
β’1Control 0 dpe; ΔRGG-hFMRP OE 0 dpeNot normalKruskal–Wallis test14; 80.1; 0.1Mean rank diff = −1.634p > 0.9999
β’1Control 0 dpe; Rg OE 0 dpeNot normalKruskal–Wallis test14; 140.1; 0.4Mean rank diff = −9.179p > 0.9999
β’1Control 0 dpe; Meng-Po OE 0 dpeNot normalKruskal–Wallis test14; 160.1; 138.9Mean rank diff = −50.82p < 0.0001
β’1Control 7 dpe; dFMRP OE 7 dpeNormalBrown–Forsythe, Welch ANOVA test12; 1853.6; 19.6t = 5.13 DF = 20.78p = 0.0002
β’1Control 7 dpe; hFMRP OE 7 dpeNormalBrown–Forsythe, Welch ANOVA test12; 1153.6; 60.5t = 0.76 DF = 19.17p = 0.9426
β’1Control 7 dpe; ΔRGG-hFMRP OE 7 dpeNormalBrown–Forsythe, Welch ANOVA test12; 1853.6; 7.1t = 8.31 DF = 12.07p < 0.0001
β’1Control 7 dpe; Rg OE 7 dpeNormalBrown–Forsythe, Welch ANOVA test12; 1653.6; 25.7t = 4.55 DF = 16.56p = 0.0014
β’1Control 7 dpe; Meng-Po OE 7 dpeNormalBrown–Forsythe, Welch ANOVA test12; 2253.6; 251.9t = 13.07 DF = 26.60p <0.0001
γ3Control 0 dpe; Meng-Po OE 0 dpeNot normalMann–Whitney test14; 160.0; 99.3U = 14p <0.0001
γ3Control 7 dpe; Meng-Po OE 7 dpeNot normalMann–Whitney test12; 160.4; 291.8U = 0p <0.0001
α’1Control 0 dpe; meng-po RNAi 0 dpeNot normalKruskal–Wallis test10; 131.0; 0.5Mean rank diff = 2.212p > 0.9999
α’1Control 0 dpe; control 7 dpeNot normalKruskal–Wallis test10; 171.0; 31.7Mean rank diff = −24.75p = 0.0004
α’1Control 0 dpe; meng-po RNAi 7 dpeNot normalKruskal–Wallis test10 ; 161.0; 0.3Mean rank diff = 6.125p > 0.9999
α’1meng-po RNAi 0 dpe; control 7 dpeNot normalKruskal–Wallis test13; 170.5; 31.7Mean rank diff = −26.96p < 0.0001
α’1meng-po RNAi 0 dpe; meng-po RNAi 7 dpeNot normalKruskal–Wallis test13; 160.5; 0.3Mean rank diff = 3.913p > 0.9999
α’1Control 7 dpe; meng-po RNAi 7 dpeNot normalKruskal–Wallis test17; 1631.7; 0.3Mean rank diff = 30.88p < 0.0001
β’1Control 0 dpe; meng-po RNAi 0 dpeNot normalKruskal–Wallis test10; 130.8; 0.3Mean rank diff = 7.138p > 0.9999
β’1Control 0 dpe; control 7 dpeNot normalKruskal–Wallis test10; 170.8; 41.7Mean rank diff = −23.90p = 0.0007
β’1Control 0 dpe; meng-po RNAi 7 dpeNot normalKruskal–Wallis test10 ; 160.8; 0.6Mean rank diff = 4.194p > 0.9999
β’1meng-po RNAi 0 dpe; control 7 dpeNot normalKruskal–Wallis test13; 170.3; 41.7Mean rank diff = −31.04p < 0.0001
β’1meng-po RNAi 0 dpe; meng-po RNAi 7 dpeNot normalKruskal–Wallis test13; 160.3; 0.6Mean rank diff = −2.945p > 0.9999
β’1Control 7 dpe; meng-po RNAi 7 dpeNot normalKruskal–Wallis test17; 1641.7; 0.6Mean rank diff = 28.09p < 0.0001
α’1shibirets 7 dpe 20°C; 25°CNormalBrown–Forsythe, Welch ANOVA test15; 147.5; 25.5t = 3.74 DF = 16.00p = 0.0053
α’1shibirets 7 dpe 20°C; 33°CNormalBrown–Forsythe, Welch ANOVA test15; 177.5; 62.2t = 11.90 DF =20.01p < 0.0001
α’1shibirets 7 dpe 25°C; 33°CNormalBrown–Forsythe, Welch ANOVA test14; 1725.5; 62.2t = 5.844 DF = 28.31p < 0.0001
β’1shibirets 7 dpe 20°C; 25°CNot normalKruskal–Wallis test16; 1418.8; 25.6Mean rank diff = −3.808p > 0.9999
β’1shibirets 7 dpe 20°C; 33°CNot normalKruskal–Wallis test16; 1718.8; 66.9Mean rank diff = −24.08p < 0.0001
β’1shibirets 7 dpe 25°C; 33°CNot normalKruskal–Wallis test14; 1725.6; 66.9Mean rank diff = −20.27p = 0.0001
α2shibirets 7 dpe 20°C; 25°CNormalBrown–Forsythe, Welch ANOVA test16; 1311.1; 53.8t = 9.33 DF = 16.03p <0.0001
α2shibirets 7 dpe 20°C; 33°CNormalBrown–Forsythe, Welch ANOVA test16; 1711.1; 9.3t = 0.67 DF = 30.76p = 0.8747
α2shibirets 7 dpe 25°C; 33°CNormalBrown–Forsythe, Welch ANOVA test13; 1753.8; 9.3t = 9.53 DF = 17.13p < 0.0001
γ1shibirets 7 dpe 20°C; 25°CNot normalKruskal–Wallis test16; 141.9; 0.9Mean rank diff = 4.353p > 0.9999
γ1shibirets 7 dpe 20°C; 33°CNot normalKruskal–Wallis test16; 171.9; 101.8Mean rank diff = −21.47p < 0.0001
γ1shibirets 7 dpe 25°C; 33°CNot normalKruskal–Wallis test14; 170.9; 101.8Mean rank diff = −25.82p < 0.0001
α2shibirets 7 dpe 20°C; 7 dpe 20°C, 3 h 33°CNot normalMann–Whitney test30; 263.6; 26.6U = 82.50p <0.0001
α’1UAS-TNT 0 dpe; UAS-TNT 7 dpeNot normalMann–Whitney test32; 571.2; 72.4U = 0p <0.0001
β’1UAS-TNT 0 dpe; UAS-TNT 7 dpeNot normalMann–Whitney test32; 570.5; 75.2U = 3.50p < 0.0001
γ1UAS-TNT 0 dpe; UAS-TNT 7 dpeNot normalMann–Whitney test31; 570.6; 18.5U = 165.50p < 0.0001
α'3UAS-TNT 0 dpe; UAS-TNT 7 dpeNot normalMann–Whitney test29; 540.3; 30.1U = 154p < 0.0001
α3UAS-TNT 0 dpe; UAS-TNT 7 dpeNot normalMann–Whitney test20; 490.2; 66.8U = 0p < 0.0001
β’2mUAS-TNT 0 dpe; UAS-TNT 7 dpeNot normalMann–Whitney test31; 570.6; 44.1U = 0p < 0.0001
β2UAS-TNT 0 dpe; UAS-TNT 7 dpeNot normalMann–Whitney test32; 580.2; 85.1U = 0p < 0.0001
α’1Control; UAS-TNT 7 dpeNot normalMann–Whitney test68; 5735.9; 72.4U = 451.50p < 0.0001
β’1Control; UAS-TNT 7 dpeNormalUnpaired two-tailed t test72; 5770.4; 75.2t = 0.83 df = 75.77p = 0.41
α’1 and
β’1		α’1 UAS-TNT 7 dpe; β’1 UAS-TNT 7 dpeNormalUnpaired two-tailed t test57; 5772.4; 75.2t =0.43 df = 101.59p = 0.67