Primers used for point mutations on T148 corresponding to the phosphomimetic (T148D) and the nonphosphorylatable (T148A) analog of αA-crystallin
| Protein | Primers |
|---|---|
| T148A | 5'-gcatccaggccagcctggatcttgggg-3' |
| 5'-ccccaagatccaggctggcctggatgc-3' | |
| T148D | 5'-gtggcatccaggccatcctggatcttggggcc-3' |
| 5'-ggccccaagatccaggatggcctggatgccac-3' |